Thiamine preserves mitochondrial function in a rat model of traumatic brain injury, preventing inactivation of the 2-oxoglutarate dehydrogenase complex

Research output: Contribution to journalJournal articleResearchpeer-review

  • Mkrtchyan, Garik
  • Muammer Üçal
  • Andrea Müllebner
  • Sergiu Dumitrescu
  • Martina Kames
  • Rudolf Moldzio
  • Marek Molcanyi
  • Samuel Schaefer
  • Adelheid Weidinger
  • Ute Schaefer
  • Juergen Hescheler
  • Johanna Catharina Duvigneau
  • Heinz Redl
  • Victoria I. Bunik
  • Andrey V. Kozlov

Background and purpose: Based on the fact that traumatic brain injury is associated with mitochondrial dysfunction we aimed at localization of mitochondrial defect and attempted to correct it by thiamine. Experimental approach: Interventional controlled experimental animal study was used. Adult male Sprague-Dawley rats were subjected to lateral fluid percussion traumatic brain injury. Thiamine was administered 1 h prior to trauma; cortex was extracted for analysis 4 h and 3 d after trauma. Key results: Increased expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor receptor 1 (TNF-R1) by 4 h was accompanied by a decrease in mitochondrial respiration with glutamate but neither with pyruvate nor succinate. Assays of TCA cycle flux-limiting 2-oxoglutarate dehydrogenase complex (OGDHC) and functionally linked enzymes (glutamate dehydrogenase, glutamine synthetase, pyruvate dehydrogenase, malate dehydrogenase and malic enzyme) indicated that only OGDHC activity was decreased. Application of the OGDHC coenzyme precursor thiamine rescued the activity of OGDHC and restored mitochondrial respiration. These effects were not mediated by changes in the expression of the OGDHC sub-units (E1k and E3), suggesting post-translational mechanism of thiamine effects. By the third day after TBI, thiamine treatment also decreased expression of TNF-R1. Specific markers of unfolded protein response did not change in response to thiamine. Conclusion and implications: Our data point to OGDHC as a major site of damage in mitochondria upon traumatic brain injury, which is associated with neuroinflammation and can be corrected by thiamine. Further studies are required to evaluate the pathological impact of these findings in clinical settings.

Original languageEnglish
JournalBiochimica et Biophysica Acta - Bioenergetics
Issue number9
Pages (from-to)925-931
Number of pages7
Publication statusPublished - 2018
Externally publishedYes

    Research areas

  • 2-Oxoglutarate dehydrogenase complex, Mitochondria, Neuroinflammation, TCA cycle, Thiamine, Traumatic brain injury (TBI)

ID: 201429213