The role of the 5′ nontranslated regions of the fusion protein mRNAs of canine distemper virus and rinderpest virus
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
The role of the 5′ nontranslated regions of the fusion protein mRNAs of canine distemper virus and rinderpest virus. / Evans, Sharon A.; Belsham, Graham J.; Barrett, Thomas.
In: Virology, Vol. 177, No. 1, 07.1990, p. 317-323.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - The role of the 5′ nontranslated regions of the fusion protein mRNAs of canine distemper virus and rinderpest virus
AU - Evans, Sharon A.
AU - Belsham, Graham J.
AU - Barrett, Thomas
N1 - Funding Information: We thank Dr. B. Moss (NIH) for the gift of vTF7-3 and Dr. B. Rima (QUB, Northern Ireland) for the CDV antiserum. The work was supported in part by Wellcome Trust Grant 14294/l .5 (T.B. and S.A.E.). We also thank L. Goatley and J. K. Brangwyn fortechnical assistance.
PY - 1990/7
Y1 - 1990/7
N2 - The mRNAs which code for the fusion proteins of the morbilliviruses (measles virus, canine distemper virus, and rinderpest virus) have unusually long 5′ untranslated regions (UTRs) which are GC-rich and are capable of folding into extensive secondary structures. In measles virus the first AUG codons in the fusion (F) protein mRNA are in close proximity at nucleotide positions 574 and 583 and protein translation is initiated at the second position. In the canine distemper virus (CDV) and rinderpest virus (RPV) F gene transcripts the analogous initiation codons are preceded by several other AUG codons many nucleotides upstream either in the same reading frame or at the beginning of other short open reading frames. We have studied the effect of deleting these upstream regions on the production of the fusion proteins of both CDV and RPV from cDNA constructs. Within the cells the presence of these regions enhances the production of the F protein while, in contrast, the production of the authentic F protein from in vitro translations using RNA transcripts is inhibited by these sequences.
AB - The mRNAs which code for the fusion proteins of the morbilliviruses (measles virus, canine distemper virus, and rinderpest virus) have unusually long 5′ untranslated regions (UTRs) which are GC-rich and are capable of folding into extensive secondary structures. In measles virus the first AUG codons in the fusion (F) protein mRNA are in close proximity at nucleotide positions 574 and 583 and protein translation is initiated at the second position. In the canine distemper virus (CDV) and rinderpest virus (RPV) F gene transcripts the analogous initiation codons are preceded by several other AUG codons many nucleotides upstream either in the same reading frame or at the beginning of other short open reading frames. We have studied the effect of deleting these upstream regions on the production of the fusion proteins of both CDV and RPV from cDNA constructs. Within the cells the presence of these regions enhances the production of the F protein while, in contrast, the production of the authentic F protein from in vitro translations using RNA transcripts is inhibited by these sequences.
UR - http://www.scopus.com/inward/record.url?scp=0025289847&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(90)90486-B
DO - 10.1016/0042-6822(90)90486-B
M3 - Journal article
C2 - 2353458
AN - SCOPUS:0025289847
VL - 177
SP - 317
EP - 323
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -
ID: 381223646