The role of proteoglycans in cancer metastasis and circulating tumor cell analysis

Research output: Contribution to journalReviewResearchpeer-review

Standard

The role of proteoglycans in cancer metastasis and circulating tumor cell analysis. / Ahrens, Theresa D.; Bang-Christensen, Sara R.; Jørgensen, Amalie M.; Løppke, Caroline; Spliid, Charlotte B.; Sand, Nicolai T.; Clausen, Thomas M.; Salanti, Ali; Agerbæk, Mette O.

In: Frontiers in Cell and Developmental Biology, Vol. 8, 749, 2020.

Research output: Contribution to journalReviewResearchpeer-review

Harvard

Ahrens, TD, Bang-Christensen, SR, Jørgensen, AM, Løppke, C, Spliid, CB, Sand, NT, Clausen, TM, Salanti, A & Agerbæk, MO 2020, 'The role of proteoglycans in cancer metastasis and circulating tumor cell analysis', Frontiers in Cell and Developmental Biology, vol. 8, 749. https://doi.org/10.3389/fcell.2020.00749

APA

Ahrens, T. D., Bang-Christensen, S. R., Jørgensen, A. M., Løppke, C., Spliid, C. B., Sand, N. T., Clausen, T. M., Salanti, A., & Agerbæk, M. O. (2020). The role of proteoglycans in cancer metastasis and circulating tumor cell analysis. Frontiers in Cell and Developmental Biology, 8, [749]. https://doi.org/10.3389/fcell.2020.00749

Vancouver

Ahrens TD, Bang-Christensen SR, Jørgensen AM, Løppke C, Spliid CB, Sand NT et al. The role of proteoglycans in cancer metastasis and circulating tumor cell analysis. Frontiers in Cell and Developmental Biology. 2020;8. 749. https://doi.org/10.3389/fcell.2020.00749

Author

Ahrens, Theresa D. ; Bang-Christensen, Sara R. ; Jørgensen, Amalie M. ; Løppke, Caroline ; Spliid, Charlotte B. ; Sand, Nicolai T. ; Clausen, Thomas M. ; Salanti, Ali ; Agerbæk, Mette O. / The role of proteoglycans in cancer metastasis and circulating tumor cell analysis. In: Frontiers in Cell and Developmental Biology. 2020 ; Vol. 8.

Bibtex

@article{0e3f633038c04bf49b5a7ba459fc5628,
title = "The role of proteoglycans in cancer metastasis and circulating tumor cell analysis",
abstract = "Circulating tumor cells (CTCs) are accessible by liquid biopsies via an easy blood draw. They represent not only the primary tumor site, but also potential metastatic lesions, and could thus be an attractive supplement for cancer diagnostics. However, the analysis of rare CTCs in billions of normal blood cells is still technically challenging and novel specific CTC markers are needed. The formation of metastasis is a complex process supported by numerous molecular alterations, and thus novel CTC markers might be found by focusing on this process. One example of this is specific changes in the cancer cell glycocalyx, which is a network on the cell surface composed of carbohydrate structures. Proteoglycans are important glycocalyx components and consist of a protein core and covalently attached long glycosaminoglycan chains. A few CTC assays have already utilized proteoglycans for both enrichment and analysis of CTCs. Nonetheless, the biological function of proteoglycans on clinical CTCs has not been studied in detail so far. Therefore, the present review describes proteoglycan functions during the metastatic cascade to highlight their importance to CTCs. We also outline current approaches for CTC assays based on targeting proteoglycans by their protein cores or their glycosaminoglycan chains. Lastly, we briefly discuss important technical aspects, which should be considered for studying proteoglycans.",
keywords = "cancer, circulating tumor cells, diagnostic, glycosaminoglycan, liquid biopsy, metastasis, proteoglycan, VAR2CSA",
author = "Ahrens, {Theresa D.} and Bang-Christensen, {Sara R.} and J{\o}rgensen, {Amalie M.} and Caroline L{\o}ppke and Spliid, {Charlotte B.} and Sand, {Nicolai T.} and Clausen, {Thomas M.} and Ali Salanti and Agerb{\ae}k, {Mette O.}",
year = "2020",
doi = "10.3389/fcell.2020.00749",
language = "English",
volume = "8",
journal = "Frontiers in Cell and Developmental Biology",
issn = "2296-634X",
publisher = "Frontiers Media",

}

RIS

TY - JOUR

T1 - The role of proteoglycans in cancer metastasis and circulating tumor cell analysis

AU - Ahrens, Theresa D.

AU - Bang-Christensen, Sara R.

AU - Jørgensen, Amalie M.

AU - Løppke, Caroline

AU - Spliid, Charlotte B.

AU - Sand, Nicolai T.

AU - Clausen, Thomas M.

AU - Salanti, Ali

AU - Agerbæk, Mette O.

PY - 2020

Y1 - 2020

N2 - Circulating tumor cells (CTCs) are accessible by liquid biopsies via an easy blood draw. They represent not only the primary tumor site, but also potential metastatic lesions, and could thus be an attractive supplement for cancer diagnostics. However, the analysis of rare CTCs in billions of normal blood cells is still technically challenging and novel specific CTC markers are needed. The formation of metastasis is a complex process supported by numerous molecular alterations, and thus novel CTC markers might be found by focusing on this process. One example of this is specific changes in the cancer cell glycocalyx, which is a network on the cell surface composed of carbohydrate structures. Proteoglycans are important glycocalyx components and consist of a protein core and covalently attached long glycosaminoglycan chains. A few CTC assays have already utilized proteoglycans for both enrichment and analysis of CTCs. Nonetheless, the biological function of proteoglycans on clinical CTCs has not been studied in detail so far. Therefore, the present review describes proteoglycan functions during the metastatic cascade to highlight their importance to CTCs. We also outline current approaches for CTC assays based on targeting proteoglycans by their protein cores or their glycosaminoglycan chains. Lastly, we briefly discuss important technical aspects, which should be considered for studying proteoglycans.

AB - Circulating tumor cells (CTCs) are accessible by liquid biopsies via an easy blood draw. They represent not only the primary tumor site, but also potential metastatic lesions, and could thus be an attractive supplement for cancer diagnostics. However, the analysis of rare CTCs in billions of normal blood cells is still technically challenging and novel specific CTC markers are needed. The formation of metastasis is a complex process supported by numerous molecular alterations, and thus novel CTC markers might be found by focusing on this process. One example of this is specific changes in the cancer cell glycocalyx, which is a network on the cell surface composed of carbohydrate structures. Proteoglycans are important glycocalyx components and consist of a protein core and covalently attached long glycosaminoglycan chains. A few CTC assays have already utilized proteoglycans for both enrichment and analysis of CTCs. Nonetheless, the biological function of proteoglycans on clinical CTCs has not been studied in detail so far. Therefore, the present review describes proteoglycan functions during the metastatic cascade to highlight their importance to CTCs. We also outline current approaches for CTC assays based on targeting proteoglycans by their protein cores or their glycosaminoglycan chains. Lastly, we briefly discuss important technical aspects, which should be considered for studying proteoglycans.

KW - cancer

KW - circulating tumor cells

KW - diagnostic

KW - glycosaminoglycan

KW - liquid biopsy

KW - metastasis

KW - proteoglycan

KW - VAR2CSA

U2 - 10.3389/fcell.2020.00749

DO - 10.3389/fcell.2020.00749

M3 - Review

C2 - 32984308

AN - SCOPUS:85090503015

VL - 8

JO - Frontiers in Cell and Developmental Biology

JF - Frontiers in Cell and Developmental Biology

SN - 2296-634X

M1 - 749

ER -

ID: 249106503