The Mobilome: Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Isolation, sequencing, and analysis of circular genetic elements bring new insights to mobile genetic elements related to microbial ecology. One method used to study circular plasmids, viruses, and other elements is called the mobilome method. The mobilome method presented here is an unamplified mobilome approach allowing fast isolation of circular DNA elements from a variety of samples followed by directly building unamplified Illumina-compatible sequencing libraries using enzymatic tagging and fragmentation. Several methods for bioinformatic analysis of mobilome data are also suggested.
Original language | English |
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Title of host publication | Horizontal Gene Transfer : Methods and Protocols |
Editors | Fernando de la Cruz |
Publisher | Humana Press |
Publication date | 2020 |
Pages | 253-264 |
Chapter | 18 |
ISBN (Print) | 978-1-4939-9876-0 |
ISBN (Electronic) | 978-1-4939-9877-7 |
DOIs | |
Publication status | Published - 2020 |
Series | Methods in Molecular Biology |
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Volume | 2075 |
ISSN | 1064-3745 |
- Circular DNA elements, Horizontal gene transfer, Metagenomics, MGE, Mobile genetic elements, Mobilome, Plasmid, Plasmidome
Research areas
ID: 234151105