The interaction of antimicrobial peptides with the membrane and intracellular targets of Staphylococcus aureus investigated by ATP leakage, DNA-binding analysis, and the expression of a LexA-controlled gene, recA
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Standard
The interaction of antimicrobial peptides with the membrane and intracellular targets of Staphylococcus aureus investigated by ATP leakage, DNA-binding analysis, and the expression of a LexA-controlled gene, recA. / Gottschalk, Sanne; Thomsen, Line Elnif.
Antimicrobial Peptides. Vol. 1548 Humana Press, 2017. p. 297-305 (Methods in Molecular Biology, Vol. 1548).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - CHAP
T1 - The interaction of antimicrobial peptides with the membrane and intracellular targets of Staphylococcus aureus investigated by ATP leakage, DNA-binding analysis, and the expression of a LexA-controlled gene, recA
AU - Gottschalk, Sanne
AU - Thomsen, Line Elnif
PY - 2017
Y1 - 2017
N2 - The analysis of how antimicrobial peptides (AMPs) interact with bacterial membranes and intracellular targets is important for our understanding of how these molecules affect bacteria. Increased knowledge may aid the design of AMPs that work on their target bacterium without inducing bacterial resistance. Here, we describe different methods to investigate the mode of action of peptides against the Gram-positive bacterium Staphylococcus aureus. ATP leakage analysis can be used to evaluate the ability of AMPs to perturb bacteria. DNA-binding and SOS response induction can be analyzed to investigate intracellular targets.
AB - The analysis of how antimicrobial peptides (AMPs) interact with bacterial membranes and intracellular targets is important for our understanding of how these molecules affect bacteria. Increased knowledge may aid the design of AMPs that work on their target bacterium without inducing bacterial resistance. Here, we describe different methods to investigate the mode of action of peptides against the Gram-positive bacterium Staphylococcus aureus. ATP leakage analysis can be used to evaluate the ability of AMPs to perturb bacteria. DNA-binding and SOS response induction can be analyzed to investigate intracellular targets.
KW - Antimicrobial peptides
KW - ATP leakage
KW - DNA binding
KW - Membrane perturbation
KW - Mode of action
KW - SOS response
U2 - 10.1007/978-1-4939-6737-7_21
DO - 10.1007/978-1-4939-6737-7_21
M3 - Book chapter
C2 - 28013513
AN - SCOPUS:85007495047
SN - 978-1-4939-6735-3
VL - 1548
T3 - Methods in Molecular Biology
SP - 297
EP - 305
BT - Antimicrobial Peptides
PB - Humana Press
ER -
ID: 188416444