The effect of storage conditions on salivary cortisol concentrations using an enzyme immunoassay
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The effect of storage conditions on salivary cortisol concentrations using an enzyme immunoassay. / Nalla, Anjana A; Thomsen, Gerda; Knudsen, Karen Birgitte Moos; Frokjaer, Vibe G.
In: Scandinavian Journal of Clinical & Laboratory Investigation, Vol. 75, No. 1, 2015, p. 92-5.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The effect of storage conditions on salivary cortisol concentrations using an enzyme immunoassay
AU - Nalla, Anjana A
AU - Thomsen, Gerda
AU - Knudsen, Karen Birgitte Moos
AU - Frokjaer, Vibe G.
PY - 2015
Y1 - 2015
N2 - Saliva samples are easy to collect and are applicable for home-sampling, e.g. when studying HPA-axis dynamics to characterize diurnal cortisol profiles and the cortisol awakening response. However, the storing and transport conditions might be critical in the home-sampling approach. Here, we tested the stability of saliva cortisol in samples stored at different temperatures and after repeated thawing-freezing cycles when measured with an Enzyme Immuno Assay (EIA). Thirteen healthy volunteers, six women and seven men, mean age 31 (range 26-49) years collected saliva either in the morning hours (08:00-10:00 h) or before lunch (11:00-12:00 h). Storage at six different conditions were tested: Storage at - 18°C, - 4°C, 4°C and room temperature for 72 h. One condition tested was at - 18°C for 72 h and then kept in an envelope for 72 h with a freezing element in room temperature surroundings where after it was stored at - 80°C. The last tube was stored directly at - 80°C and served as the 'gold standard'. The saliva samples were assayed using Salivary Cortisol Diagnostic EIA. Differences in cortisol measurements between each of the five conditions and the 'gold standard' (- 80°C) were evaluated by one-sample t-test. No significant differences were observed. This indicates that an EIA method can be used reliably when measuring salivary cortisol samples obtained by home-sampling including a postal delivery.
AB - Saliva samples are easy to collect and are applicable for home-sampling, e.g. when studying HPA-axis dynamics to characterize diurnal cortisol profiles and the cortisol awakening response. However, the storing and transport conditions might be critical in the home-sampling approach. Here, we tested the stability of saliva cortisol in samples stored at different temperatures and after repeated thawing-freezing cycles when measured with an Enzyme Immuno Assay (EIA). Thirteen healthy volunteers, six women and seven men, mean age 31 (range 26-49) years collected saliva either in the morning hours (08:00-10:00 h) or before lunch (11:00-12:00 h). Storage at six different conditions were tested: Storage at - 18°C, - 4°C, 4°C and room temperature for 72 h. One condition tested was at - 18°C for 72 h and then kept in an envelope for 72 h with a freezing element in room temperature surroundings where after it was stored at - 80°C. The last tube was stored directly at - 80°C and served as the 'gold standard'. The saliva samples were assayed using Salivary Cortisol Diagnostic EIA. Differences in cortisol measurements between each of the five conditions and the 'gold standard' (- 80°C) were evaluated by one-sample t-test. No significant differences were observed. This indicates that an EIA method can be used reliably when measuring salivary cortisol samples obtained by home-sampling including a postal delivery.
KW - Adult
KW - Cryopreservation
KW - Enzyme-Linked Immunosorbent Assay
KW - Female
KW - Humans
KW - Hydrocortisone
KW - Male
KW - Middle Aged
KW - Saliva
U2 - 10.3109/00365513.2014.985252
DO - 10.3109/00365513.2014.985252
M3 - Journal article
C2 - 25510953
VL - 75
SP - 92
EP - 95
JO - Scandinavian Journal of Clinical & Laboratory Investigation
JF - Scandinavian Journal of Clinical & Laboratory Investigation
SN - 0036-5513
IS - 1
ER -
ID: 162872921