TY - JOUR

T1 - The circulating common gamma chain (CD132) in inflammatory bowel disease

AU - Nielsen, Ole Haagen

AU - Kirman, Irena

AU - Johnson, Kirk

AU - Giedlin, Martin

AU - Ciardelli, Thomas

PY - 1998/3/1

Y1 - 1998/3/1

N2 - Objective: Inflammatory bowel disease (IBD) is characterized by T cell activation. Activated T cells shed interleukin-2 receptors (IL-2R) in a soluble form. A positive correlation between sIL-2Rα (CD25) and disease activity is well documented in IBD, whereas IL-2Rγ (CD132) has not been investigated in this respect. Sera from 42 patients with ulcerative colitis (UC), 34 with Crohn's disease (CD), 31 healthy volunteers, and 12 patients with infectious enterocolitis were obtained. Methods: Disease activity was scored according to a semiquantitative score for UC and CD. sIL-2R α chain and γ chain were assessed by sandwich ELISA techniques using monoclonal antibodies specific for CD25 and CD132, respectively. Results: The concentration of IL-2Rα chain (CD25) was found to be median 3.8 ng/ml in healthy volunteers versus 7.0 ng/ml in UC patients (p < 0.001), and 9.6 ng/ml in CD patients (p < 0.001). With respect to IL-2Rγ (CD132), significantly higher amounts were found in CD patients: 6.6 ng/ml as compared with healthy controls < 1.0 ng/ml (p < 0.004). A Kruskal-Wallis test revealed a significant correlation between α chain and disease activity in CD (p < 0.001), and further significantly higher γ chain levels were found in active CD (p = 0.03). For UC patients, a statistically significant increase of the α chain with increasing disease activity (p < 0.01) was observed, whereas no significant changes of the y chain levels were found (p > 0.05). A difference of y chain levels were found between CD and UC in moderate and severe disease activity (p < 0.05). Further analyses revealed that mesalazine did not influence the IL-2Rα or γ concentration either in UC or in CD patients. Conclusion: An increased circulating level of the soluble common γ chain (CD132) seems to be found in CD, and an overlap exists between CD and UC.

AB - Objective: Inflammatory bowel disease (IBD) is characterized by T cell activation. Activated T cells shed interleukin-2 receptors (IL-2R) in a soluble form. A positive correlation between sIL-2Rα (CD25) and disease activity is well documented in IBD, whereas IL-2Rγ (CD132) has not been investigated in this respect. Sera from 42 patients with ulcerative colitis (UC), 34 with Crohn's disease (CD), 31 healthy volunteers, and 12 patients with infectious enterocolitis were obtained. Methods: Disease activity was scored according to a semiquantitative score for UC and CD. sIL-2R α chain and γ chain were assessed by sandwich ELISA techniques using monoclonal antibodies specific for CD25 and CD132, respectively. Results: The concentration of IL-2Rα chain (CD25) was found to be median 3.8 ng/ml in healthy volunteers versus 7.0 ng/ml in UC patients (p < 0.001), and 9.6 ng/ml in CD patients (p < 0.001). With respect to IL-2Rγ (CD132), significantly higher amounts were found in CD patients: 6.6 ng/ml as compared with healthy controls < 1.0 ng/ml (p < 0.004). A Kruskal-Wallis test revealed a significant correlation between α chain and disease activity in CD (p < 0.001), and further significantly higher γ chain levels were found in active CD (p = 0.03). For UC patients, a statistically significant increase of the α chain with increasing disease activity (p < 0.01) was observed, whereas no significant changes of the y chain levels were found (p > 0.05). A difference of y chain levels were found between CD and UC in moderate and severe disease activity (p < 0.05). Further analyses revealed that mesalazine did not influence the IL-2Rα or γ concentration either in UC or in CD patients. Conclusion: An increased circulating level of the soluble common γ chain (CD132) seems to be found in CD, and an overlap exists between CD and UC.

UR - http://www.scopus.com/inward/record.url?scp=0032033981&partnerID=8YFLogxK

U2 - 10.1111/j.1572-0241.1998.00323.x

DO - 10.1111/j.1572-0241.1998.00323.x

M3 - Journal article

C2 - 9517632

AN - SCOPUS:0032033981

VL - 93

SP - 323

EP - 328

JO - The American Journal of Gastroenterology

JF - The American Journal of Gastroenterology

SN - 0002-9270

IS - 3

ER -