The activity of satellite cells and myonuclei following 8 weeks of strength training in young men with suppressed testosterone levels
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The activity of satellite cells and myonuclei following 8 weeks of strength training in young men with suppressed testosterone levels. / Kvorning, T; Kadi, F; Schjerling, P.; Andersen, M.; Brixen, K.; Suetta, C; Madsen, K.
In: Acta Physiologica (Print), Vol. 213, No. 3, 03.2015, p. 676-87.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The activity of satellite cells and myonuclei following 8 weeks of strength training in young men with suppressed testosterone levels
AU - Kvorning, T
AU - Kadi, F
AU - Schjerling, P.
AU - Andersen, M.
AU - Brixen, K.
AU - Suetta, C
AU - Madsen, K
N1 - © 2014 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.
PY - 2015/3
Y1 - 2015/3
N2 - AIM: To investigate how suppression of endogenous testosterone during an 8-week strength training period influences the activity of satellite cells and myonuclei.METHODS: Twenty-two moderately trained young men participated in this randomized, placebo-controlled, and double-blinded intervention study. The participants were randomized to treatment with a GnRH analogue, goserelin (n = 12), which suppresses testosterone or placebo (n = 10) for 12 weeks. The strength training period of 8 weeks started after 4 weeks of treatment and included exercises for all major muscles. Biopsies were obtained from the mid-portion of the vastus lateralis muscle.RESULTS: Testosterone resting level in goserelin was 10-20 times lower compared with placebo, and the training-induced increase in the level of testosterone was abolished in goserelin. Training increased satellite cells number in type II fibres by 20% in placebo and by 52% in goserelin (P < 0.01), whereas the myonuclear number significantly increased by 12% in type II fibres in placebo and remained unchanged in goserelin (P < 0.05). No changes in satellite cells and myonuclei were seen in type I fibres in either group. Data from the microarray analysis indicated that low testosterone affects the bone morphogenetic proteins signalling, which might regulate proliferation vs. differentiation of satellite cells.CONCLUSION: Eight weeks of strength training enhances the myonuclear number in type II fibres, and this is largely blocked by the suppression of testosterone. The data indicate that low testosterone levels could reduce the differentiation of satellite cells to myonuclei via the bone morphogenetic proteins signalling pathway, resulting in reduced increases in lean leg mass.
AB - AIM: To investigate how suppression of endogenous testosterone during an 8-week strength training period influences the activity of satellite cells and myonuclei.METHODS: Twenty-two moderately trained young men participated in this randomized, placebo-controlled, and double-blinded intervention study. The participants were randomized to treatment with a GnRH analogue, goserelin (n = 12), which suppresses testosterone or placebo (n = 10) for 12 weeks. The strength training period of 8 weeks started after 4 weeks of treatment and included exercises for all major muscles. Biopsies were obtained from the mid-portion of the vastus lateralis muscle.RESULTS: Testosterone resting level in goserelin was 10-20 times lower compared with placebo, and the training-induced increase in the level of testosterone was abolished in goserelin. Training increased satellite cells number in type II fibres by 20% in placebo and by 52% in goserelin (P < 0.01), whereas the myonuclear number significantly increased by 12% in type II fibres in placebo and remained unchanged in goserelin (P < 0.05). No changes in satellite cells and myonuclei were seen in type I fibres in either group. Data from the microarray analysis indicated that low testosterone affects the bone morphogenetic proteins signalling, which might regulate proliferation vs. differentiation of satellite cells.CONCLUSION: Eight weeks of strength training enhances the myonuclear number in type II fibres, and this is largely blocked by the suppression of testosterone. The data indicate that low testosterone levels could reduce the differentiation of satellite cells to myonuclei via the bone morphogenetic proteins signalling pathway, resulting in reduced increases in lean leg mass.
KW - Adult
KW - Age Factors
KW - Biopsy
KW - Double-Blind Method
KW - Gene Expression Profiling
KW - Gene Expression Regulation
KW - Goserelin
KW - Humans
KW - Male
KW - Muscle Fibers, Fast-Twitch
KW - Quadriceps Muscle
KW - RNA, Messenger
KW - Resistance Training
KW - Satellite Cells, Skeletal Muscle
KW - Sex Factors
KW - Testosterone
KW - Time Factors
KW - Young Adult
U2 - 10.1111/apha.12404
DO - 10.1111/apha.12404
M3 - Journal article
C2 - 25294097
VL - 213
SP - 676
EP - 687
JO - Acta Physiologica
JF - Acta Physiologica
SN - 1748-1708
IS - 3
ER -
ID: 162445810