Targeted 3' processing of antisense transcripts triggers Arabidopsis FLC chromatin silencing

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Targeted 3' processing of antisense transcripts triggers Arabidopsis FLC chromatin silencing. / Liu, Fuquan; Marquardt, Sebastian; Lister, Clare; Swiezewski, Szymon; Dean, Caroline.

In: Science (New York, N.Y.), Vol. 327, No. 5961, 2010, p. 94-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Liu, F, Marquardt, S, Lister, C, Swiezewski, S & Dean, C 2010, 'Targeted 3' processing of antisense transcripts triggers Arabidopsis FLC chromatin silencing', Science (New York, N.Y.), vol. 327, no. 5961, pp. 94-7. https://doi.org/10.1126/science.1180278

APA

Liu, F., Marquardt, S., Lister, C., Swiezewski, S., & Dean, C. (2010). Targeted 3' processing of antisense transcripts triggers Arabidopsis FLC chromatin silencing. Science (New York, N.Y.), 327(5961), 94-7. https://doi.org/10.1126/science.1180278

Vancouver

Liu F, Marquardt S, Lister C, Swiezewski S, Dean C. Targeted 3' processing of antisense transcripts triggers Arabidopsis FLC chromatin silencing. Science (New York, N.Y.). 2010;327(5961):94-7. https://doi.org/10.1126/science.1180278

Author

Liu, Fuquan ; Marquardt, Sebastian ; Lister, Clare ; Swiezewski, Szymon ; Dean, Caroline. / Targeted 3' processing of antisense transcripts triggers Arabidopsis FLC chromatin silencing. In: Science (New York, N.Y.). 2010 ; Vol. 327, No. 5961. pp. 94-7.

Bibtex

@article{9e4b9dbc5e5a4dc88938b7678371de42,
title = "Targeted 3' processing of antisense transcripts triggers Arabidopsis FLC chromatin silencing",
abstract = "Noncoding RNA is emerging as an important regulator of gene expression in many organisms. We are characterizing RNA-mediated chromatin silencing of the Arabidopsis major floral repressor gene, FLC. Through suppressor mutagenesis, we identify a requirement for CstF64 and CstF77, two conserved RNA 3'-end-processing factors, in FLC silencing. However, FLC sense transcript 3' processing is not affected in the mutants. Instead, CstF64 and CstF77 are required for 3' processing of FLC antisense transcripts. A specific RNA-binding protein directs their activity to a proximal antisense polyadenylation site. This targeted processing triggers localized histone demethylase activity and results in reduced FLC sense transcription. Targeted 3' processing of antisense transcripts may be a common mechanism triggering transcriptional silencing of the corresponding sense gene.",
keywords = "Arabidopsis, Arabidopsis Proteins, Chromatin, Cleavage Stimulation Factor, Epistasis, Genetic, Flowers, Gene Expression Regulation, Plant, Gene Silencing, Histone Deacetylases, MADS Domain Proteins, Models, Genetic, Polyadenylation, RNA Processing, Post-Transcriptional, RNA, Antisense, RNA, Plant, RNA-Binding Proteins, Suppression, Genetic, Transcription, Genetic, mRNA Cleavage and Polyadenylation Factors, Journal Article, Research Support, Non-U.S. Gov't",
author = "Fuquan Liu and Sebastian Marquardt and Clare Lister and Szymon Swiezewski and Caroline Dean",
year = "2010",
doi = "10.1126/science.1180278",
language = "English",
volume = "327",
pages = "94--7",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5961",

}

RIS

TY - JOUR

T1 - Targeted 3' processing of antisense transcripts triggers Arabidopsis FLC chromatin silencing

AU - Liu, Fuquan

AU - Marquardt, Sebastian

AU - Lister, Clare

AU - Swiezewski, Szymon

AU - Dean, Caroline

PY - 2010

Y1 - 2010

N2 - Noncoding RNA is emerging as an important regulator of gene expression in many organisms. We are characterizing RNA-mediated chromatin silencing of the Arabidopsis major floral repressor gene, FLC. Through suppressor mutagenesis, we identify a requirement for CstF64 and CstF77, two conserved RNA 3'-end-processing factors, in FLC silencing. However, FLC sense transcript 3' processing is not affected in the mutants. Instead, CstF64 and CstF77 are required for 3' processing of FLC antisense transcripts. A specific RNA-binding protein directs their activity to a proximal antisense polyadenylation site. This targeted processing triggers localized histone demethylase activity and results in reduced FLC sense transcription. Targeted 3' processing of antisense transcripts may be a common mechanism triggering transcriptional silencing of the corresponding sense gene.

AB - Noncoding RNA is emerging as an important regulator of gene expression in many organisms. We are characterizing RNA-mediated chromatin silencing of the Arabidopsis major floral repressor gene, FLC. Through suppressor mutagenesis, we identify a requirement for CstF64 and CstF77, two conserved RNA 3'-end-processing factors, in FLC silencing. However, FLC sense transcript 3' processing is not affected in the mutants. Instead, CstF64 and CstF77 are required for 3' processing of FLC antisense transcripts. A specific RNA-binding protein directs their activity to a proximal antisense polyadenylation site. This targeted processing triggers localized histone demethylase activity and results in reduced FLC sense transcription. Targeted 3' processing of antisense transcripts may be a common mechanism triggering transcriptional silencing of the corresponding sense gene.

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Chromatin

KW - Cleavage Stimulation Factor

KW - Epistasis, Genetic

KW - Flowers

KW - Gene Expression Regulation, Plant

KW - Gene Silencing

KW - Histone Deacetylases

KW - MADS Domain Proteins

KW - Models, Genetic

KW - Polyadenylation

KW - RNA Processing, Post-Transcriptional

KW - RNA, Antisense

KW - RNA, Plant

KW - RNA-Binding Proteins

KW - Suppression, Genetic

KW - Transcription, Genetic

KW - mRNA Cleavage and Polyadenylation Factors

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1126/science.1180278

DO - 10.1126/science.1180278

M3 - Journal article

C2 - 19965720

VL - 327

SP - 94

EP - 97

JO - Science

JF - Science

SN - 0036-8075

IS - 5961

ER -

ID: 183164664