Successful STR and SNP typing of FTA Card samples with low amounts of DNA after DNA extraction using a Qiagen BioRobot® EZ1 Workstation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Successful STR and SNP typing of FTA Card samples with low amounts of DNA after DNA extraction using a Qiagen BioRobot® EZ1 Workstation. / Rockenbauer, Eszter; Børsting, Claus; Stangegaard, Michael; Frank-Hansen, Rune; Morling, Niels.

In: Forensic Science International: Genetics Supplement Series, Vol. 2, No. 1, 2009, p. 83-84.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rockenbauer, E, Børsting, C, Stangegaard, M, Frank-Hansen, R & Morling, N 2009, 'Successful STR and SNP typing of FTA Card samples with low amounts of DNA after DNA extraction using a Qiagen BioRobot® EZ1 Workstation', Forensic Science International: Genetics Supplement Series, vol. 2, no. 1, pp. 83-84. https://doi.org/10.1016/j.fsigss.2009.08.152

APA

Rockenbauer, E., Børsting, C., Stangegaard, M., Frank-Hansen, R., & Morling, N. (2009). Successful STR and SNP typing of FTA Card samples with low amounts of DNA after DNA extraction using a Qiagen BioRobot® EZ1 Workstation. Forensic Science International: Genetics Supplement Series, 2(1), 83-84. https://doi.org/10.1016/j.fsigss.2009.08.152

Vancouver

Rockenbauer E, Børsting C, Stangegaard M, Frank-Hansen R, Morling N. Successful STR and SNP typing of FTA Card samples with low amounts of DNA after DNA extraction using a Qiagen BioRobot® EZ1 Workstation. Forensic Science International: Genetics Supplement Series. 2009;2(1):83-84. https://doi.org/10.1016/j.fsigss.2009.08.152

Author

Rockenbauer, Eszter ; Børsting, Claus ; Stangegaard, Michael ; Frank-Hansen, Rune ; Morling, Niels. / Successful STR and SNP typing of FTA Card samples with low amounts of DNA after DNA extraction using a Qiagen BioRobot® EZ1 Workstation. In: Forensic Science International: Genetics Supplement Series. 2009 ; Vol. 2, No. 1. pp. 83-84.

Bibtex

@article{87ef9fc558254909a4c9635c4749948d,
title = "Successful STR and SNP typing of FTA Card samples with low amounts of DNA after DNA extraction using a Qiagen BioRobot{\textregistered} EZ1 Workstation",
abstract = "FTA Cards (GE Healthcare) have been used for more than 4 years in Denmark for the collection of buccal cells as reference samples in crime cases. Semi-automated protocols for STR typing of DNA on punches of FTA Cards are routinely used. In average, full STR profiles were generated from approximately 95% of the FTA Cards with a standard punching protocol, while partial or no STR profile were obtained from 5% of the samples. Here, the Qiagen BioRobot{\textregistered} EZ1 Workstation (Qiagen) and the EZ1 DNA Investigator Kit (Qiagen) was used to extract DNA from 29 FTA Cards from which a complete STR profile was not generated with the standard punching protocol. All 29 samples were successfully typed with the AmpFℓSTR{\textregistered} Identifiler{\texttrademark} PCR Amplification Kit (Applied Biosystems) and with the SNPforID 49plex SNP assay. The lowest amount of DNA that resulted in complete STR and SNP profiles was 80 pg. The STR and SNP profiles were identical to those generated from another sample collected from each of the 29 individuals.",
keywords = "DNA extraction, FTA Cards, Reference samples, SNP, STR",
author = "Eszter Rockenbauer and Claus B{\o}rsting and Michael Stangegaard and Rune Frank-Hansen and Niels Morling",
year = "2009",
doi = "10.1016/j.fsigss.2009.08.152",
language = "English",
volume = "2",
pages = "83--84",
journal = "Forensic Science International: Genetics. Supplement Series",
issn = "1875-1768",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Successful STR and SNP typing of FTA Card samples with low amounts of DNA after DNA extraction using a Qiagen BioRobot® EZ1 Workstation

AU - Rockenbauer, Eszter

AU - Børsting, Claus

AU - Stangegaard, Michael

AU - Frank-Hansen, Rune

AU - Morling, Niels

PY - 2009

Y1 - 2009

N2 - FTA Cards (GE Healthcare) have been used for more than 4 years in Denmark for the collection of buccal cells as reference samples in crime cases. Semi-automated protocols for STR typing of DNA on punches of FTA Cards are routinely used. In average, full STR profiles were generated from approximately 95% of the FTA Cards with a standard punching protocol, while partial or no STR profile were obtained from 5% of the samples. Here, the Qiagen BioRobot® EZ1 Workstation (Qiagen) and the EZ1 DNA Investigator Kit (Qiagen) was used to extract DNA from 29 FTA Cards from which a complete STR profile was not generated with the standard punching protocol. All 29 samples were successfully typed with the AmpFℓSTR® Identifiler™ PCR Amplification Kit (Applied Biosystems) and with the SNPforID 49plex SNP assay. The lowest amount of DNA that resulted in complete STR and SNP profiles was 80 pg. The STR and SNP profiles were identical to those generated from another sample collected from each of the 29 individuals.

AB - FTA Cards (GE Healthcare) have been used for more than 4 years in Denmark for the collection of buccal cells as reference samples in crime cases. Semi-automated protocols for STR typing of DNA on punches of FTA Cards are routinely used. In average, full STR profiles were generated from approximately 95% of the FTA Cards with a standard punching protocol, while partial or no STR profile were obtained from 5% of the samples. Here, the Qiagen BioRobot® EZ1 Workstation (Qiagen) and the EZ1 DNA Investigator Kit (Qiagen) was used to extract DNA from 29 FTA Cards from which a complete STR profile was not generated with the standard punching protocol. All 29 samples were successfully typed with the AmpFℓSTR® Identifiler™ PCR Amplification Kit (Applied Biosystems) and with the SNPforID 49plex SNP assay. The lowest amount of DNA that resulted in complete STR and SNP profiles was 80 pg. The STR and SNP profiles were identical to those generated from another sample collected from each of the 29 individuals.

KW - DNA extraction

KW - FTA Cards

KW - Reference samples

KW - SNP

KW - STR

U2 - 10.1016/j.fsigss.2009.08.152

DO - 10.1016/j.fsigss.2009.08.152

M3 - Journal article

AN - SCOPUS:70649084046

VL - 2

SP - 83

EP - 84

JO - Forensic Science International: Genetics. Supplement Series

JF - Forensic Science International: Genetics. Supplement Series

SN - 1875-1768

IS - 1

ER -

ID: 307818865