Strategies for the capillary electrophoretic separation of indole alkaloids in Psilocybe semilanceata
Research output: Contribution to journal › Journal article › Research › peer-review
While the hallucinogenic mushrooms Psilocybe semilanceata have previously been analyzed for the indole alkaloids psilocybin and baeocystin by capillary zone electrophoresis (CZE) at pH 11.5, the present work focused on the development of an alternative and complementary capillary electrophoretic method for their identification. Owing to their structural similarity and zwitterionic nature, the compounds were difficult to resolve based on different interactions with cationic or anionic micelles. However, while the attempts with micellar electrokinetic chromatography (MEKC) were unsuccessful, rapid derivatization with propyl chloroformate and reanalysis by CZE at pH 11.5 was effective to support identification of the two indole alkaloids. Psilocin was difficult to analyze by CZE at pH 11.5 owing to comigration with the electroosmotic flow. For this compound, the pH of the running buffer was reduced to 7.2 to effectively enhance the electrophoretic mobility.
Original language | English |
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Journal | Electrophoresis |
Volume | 19 |
Issue number | 1 |
Pages (from-to) | 27-30 |
Number of pages | 4 |
ISSN | 0173-0835 |
DOIs | |
Publication status | Published - Jan 1998 |
- Capillary electrophoresis, Indole alkaloids, Psilocybe semilanceata
Research areas
ID: 231655002