Spinal dorsal horn astrocytes release GABA in response to synaptic activation

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Spinal dorsal horn astrocytes release GABA in response to synaptic activation. / Christensen, Rasmus Kordt; Delgado-Lezama, Rodolfo; Russo, Raúl E; Lind, Barbara Lykke; Alcocer, Emanuel Loeza; Rath, Martin Fredensborg; Fabbiani, Gabriela; Schmitt, Nicole; Lauritzen, Martin; Petersen, Anders Victor; Carlsen, Eva Meier; Perrier, Jean-François.

In: The Journal of Physiology, Vol. 596, No. 20, 2018, p. 4983-4994.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Christensen, RK, Delgado-Lezama, R, Russo, RE, Lind, BL, Alcocer, EL, Rath, MF, Fabbiani, G, Schmitt, N, Lauritzen, M, Petersen, AV, Carlsen, EM & Perrier, J-F 2018, 'Spinal dorsal horn astrocytes release GABA in response to synaptic activation', The Journal of Physiology, vol. 596, no. 20, pp. 4983-4994. https://doi.org/10.1113/JP276562

APA

Christensen, R. K., Delgado-Lezama, R., Russo, R. E., Lind, B. L., Alcocer, E. L., Rath, M. F., ... Perrier, J-F. (2018). Spinal dorsal horn astrocytes release GABA in response to synaptic activation. The Journal of Physiology, 596(20), 4983-4994. https://doi.org/10.1113/JP276562

Vancouver

Christensen RK, Delgado-Lezama R, Russo RE, Lind BL, Alcocer EL, Rath MF et al. Spinal dorsal horn astrocytes release GABA in response to synaptic activation. The Journal of Physiology. 2018;596(20):4983-4994. https://doi.org/10.1113/JP276562

Author

Christensen, Rasmus Kordt ; Delgado-Lezama, Rodolfo ; Russo, Raúl E ; Lind, Barbara Lykke ; Alcocer, Emanuel Loeza ; Rath, Martin Fredensborg ; Fabbiani, Gabriela ; Schmitt, Nicole ; Lauritzen, Martin ; Petersen, Anders Victor ; Carlsen, Eva Meier ; Perrier, Jean-François. / Spinal dorsal horn astrocytes release GABA in response to synaptic activation. In: The Journal of Physiology. 2018 ; Vol. 596, No. 20. pp. 4983-4994.

Bibtex

@article{e7c59e959785446faa171bff38fbca0d,
title = "Spinal dorsal horn astrocytes release GABA in response to synaptic activation",
abstract = "KEY POINTS SUMMARY: GABA is an essential molecule for sensory information processing. It is usually assumed to be released by neurons. Here we show that in the dorsal horn of the spinal cord, astrocytes respond to glutamate by releasing GABA. Our findings suggest a novel role for astrocytes in somatosensory information processing.ABSTRACT: Astrocytes participate in neuronal signalling by releasing gliotransmitters in response to neurotransmitters. We investigated if astrocytes from the dorsal horn of the spinal cord of adult red-eared turtles (Trachemys scripta elegans) release GABA in response to glutamatergic receptor activation. For this, we developed a GABA sensor consisting of HEK cells expressing GABAA receptors. By positioning the sensor recorded in the whole-cell patch-clamp configuration within the dorsal horn of a spinal cord slice, we could detect GABA in the extracellular space. Puff application of glutamate induced GABA release events with time courses that exceeded the duration of inhibitory postsynaptic currents by one order of magnitude. Because the events were neither affected by extracellular addition of nickel, cadmium and tetrodotoxin nor by removal of Ca2+ , we concluded that they originated from non-neuronal cells. Immunohistochemical staining allowed the detection of GABA in a fraction of dorsal horn astrocytes. The selective stimulation of A∂ and C fibres in a dorsal root filament induced a Ca2+ increase in astrocytes loaded with Oregon Green BAPTA. Finally, chelating Ca2+ in a single astrocyte was sufficient to prevent the GABA release evoked by glutamate. Our results indicate that glutamate triggers the release of GABA from dorsal horn astrocytes with a time course compatible with the integration of sensory inputs. This article is protected by copyright. All rights reserved.",
keywords = "astrocyte, GABA, spinal cord",
author = "Christensen, {Rasmus Kordt} and Rodolfo Delgado-Lezama and Russo, {Ra{\'u}l E} and Lind, {Barbara Lykke} and Alcocer, {Emanuel Loeza} and Rath, {Martin Fredensborg} and Gabriela Fabbiani and Nicole Schmitt and Martin Lauritzen and Petersen, {Anders Victor} and Carlsen, {Eva Meier} and Jean-Fran{\cc}ois Perrier",
note = "This article is protected by copyright. All rights reserved.",
year = "2018",
doi = "10.1113/JP276562",
language = "English",
volume = "596",
pages = "4983--4994",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "20",

}

RIS

TY - JOUR

T1 - Spinal dorsal horn astrocytes release GABA in response to synaptic activation

AU - Christensen, Rasmus Kordt

AU - Delgado-Lezama, Rodolfo

AU - Russo, Raúl E

AU - Lind, Barbara Lykke

AU - Alcocer, Emanuel Loeza

AU - Rath, Martin Fredensborg

AU - Fabbiani, Gabriela

AU - Schmitt, Nicole

AU - Lauritzen, Martin

AU - Petersen, Anders Victor

AU - Carlsen, Eva Meier

AU - Perrier, Jean-François

N1 - This article is protected by copyright. All rights reserved.

PY - 2018

Y1 - 2018

N2 - KEY POINTS SUMMARY: GABA is an essential molecule for sensory information processing. It is usually assumed to be released by neurons. Here we show that in the dorsal horn of the spinal cord, astrocytes respond to glutamate by releasing GABA. Our findings suggest a novel role for astrocytes in somatosensory information processing.ABSTRACT: Astrocytes participate in neuronal signalling by releasing gliotransmitters in response to neurotransmitters. We investigated if astrocytes from the dorsal horn of the spinal cord of adult red-eared turtles (Trachemys scripta elegans) release GABA in response to glutamatergic receptor activation. For this, we developed a GABA sensor consisting of HEK cells expressing GABAA receptors. By positioning the sensor recorded in the whole-cell patch-clamp configuration within the dorsal horn of a spinal cord slice, we could detect GABA in the extracellular space. Puff application of glutamate induced GABA release events with time courses that exceeded the duration of inhibitory postsynaptic currents by one order of magnitude. Because the events were neither affected by extracellular addition of nickel, cadmium and tetrodotoxin nor by removal of Ca2+ , we concluded that they originated from non-neuronal cells. Immunohistochemical staining allowed the detection of GABA in a fraction of dorsal horn astrocytes. The selective stimulation of A∂ and C fibres in a dorsal root filament induced a Ca2+ increase in astrocytes loaded with Oregon Green BAPTA. Finally, chelating Ca2+ in a single astrocyte was sufficient to prevent the GABA release evoked by glutamate. Our results indicate that glutamate triggers the release of GABA from dorsal horn astrocytes with a time course compatible with the integration of sensory inputs. This article is protected by copyright. All rights reserved.

AB - KEY POINTS SUMMARY: GABA is an essential molecule for sensory information processing. It is usually assumed to be released by neurons. Here we show that in the dorsal horn of the spinal cord, astrocytes respond to glutamate by releasing GABA. Our findings suggest a novel role for astrocytes in somatosensory information processing.ABSTRACT: Astrocytes participate in neuronal signalling by releasing gliotransmitters in response to neurotransmitters. We investigated if astrocytes from the dorsal horn of the spinal cord of adult red-eared turtles (Trachemys scripta elegans) release GABA in response to glutamatergic receptor activation. For this, we developed a GABA sensor consisting of HEK cells expressing GABAA receptors. By positioning the sensor recorded in the whole-cell patch-clamp configuration within the dorsal horn of a spinal cord slice, we could detect GABA in the extracellular space. Puff application of glutamate induced GABA release events with time courses that exceeded the duration of inhibitory postsynaptic currents by one order of magnitude. Because the events were neither affected by extracellular addition of nickel, cadmium and tetrodotoxin nor by removal of Ca2+ , we concluded that they originated from non-neuronal cells. Immunohistochemical staining allowed the detection of GABA in a fraction of dorsal horn astrocytes. The selective stimulation of A∂ and C fibres in a dorsal root filament induced a Ca2+ increase in astrocytes loaded with Oregon Green BAPTA. Finally, chelating Ca2+ in a single astrocyte was sufficient to prevent the GABA release evoked by glutamate. Our results indicate that glutamate triggers the release of GABA from dorsal horn astrocytes with a time course compatible with the integration of sensory inputs. This article is protected by copyright. All rights reserved.

KW - astrocyte

KW - GABA

KW - spinal cord

UR - http://www.scopus.com/inward/record.url?scp=85052822596&partnerID=8YFLogxK

U2 - 10.1113/JP276562

DO - 10.1113/JP276562

M3 - Journal article

C2 - 30079574

VL - 596

SP - 4983

EP - 4994

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - 20

ER -

ID: 201453053