SOCS5 is expressed in primary B and T lymphoid cells but is dispensable for lymphocyte production and function
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SOCS5 is expressed in primary B and T lymphoid cells but is dispensable for lymphocyte production and function. / Brender, Christine; Columbus, Ruth; Metcalf, Donald; Handman, Emanuela; Starr, Robyn; Huntington, Nick; Tarlinton, David; Ødum, Niels; Nicholson, Sandra E; Nicola, Nicos A; Hilton, Douglas J; Alexander, Warren S.
In: Molecular and Cellular Biology, Vol. 24, No. 13, 2004, p. 6094-103.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - SOCS5 is expressed in primary B and T lymphoid cells but is dispensable for lymphocyte production and function
AU - Brender, Christine
AU - Columbus, Ruth
AU - Metcalf, Donald
AU - Handman, Emanuela
AU - Starr, Robyn
AU - Huntington, Nick
AU - Tarlinton, David
AU - Ødum, Niels
AU - Nicholson, Sandra E
AU - Nicola, Nicos A
AU - Hilton, Douglas J
AU - Alexander, Warren S
N1 - Keywords: Animals; B-Lymphocytes; Cell Differentiation; Female; Hematopoiesis; Leishmania major; Leishmaniasis, Cutaneous; Lymphocyte Activation; Mice; Mice, Knockout; Protein Biosynthesis; Proteins; Suppressor of Cytokine Signaling Proteins; T-Lymphocytes; T-Lymphocytes, Helper-Inducer
PY - 2004
Y1 - 2004
N2 - Suppressors of cytokine signaling (SOCSs) are key regulators of cytokine-induced responses in hematopoietic as well as nonhematopoietic cells. SOCS1 and SOCS3 have been shown to modulate T-cell responses, whereas the roles of other SOCS family members in the regulation of lymphocyte function are less clear. Here, we report the generation of mice with a targeted disruption of the Socs5 gene. Socs5(-/-) mice were born in a normal Mendelian ratio and were healthy and fertile. We found that SOCS5 is expressed in primary B and T cells in wild-type mice. However, no abnormalities in the lymphocyte compartment were seen in SOCS5-deficient mice. We examined antigen- and cytokine-induced proliferative responses in B and T cells in the absence of SOCS5 and found no deviations from the responses seen in wild-type cells. Because SOCS5 has been implicated in Th1 differentiation, we also investigated the importance of SOCS5 in T helper cell responses. Unexpectedly, SOCS5-deficient CD4 T cells showed no abnormalities in Th1/Th2 differentiation and Socs5(-/-) mice showed normal resistance to infection with Leishmania major. Therefore, although SOCS5 is expressed in primary B and T cells, it appears to be dispensable for the regulation of lymphocyte function.
AB - Suppressors of cytokine signaling (SOCSs) are key regulators of cytokine-induced responses in hematopoietic as well as nonhematopoietic cells. SOCS1 and SOCS3 have been shown to modulate T-cell responses, whereas the roles of other SOCS family members in the regulation of lymphocyte function are less clear. Here, we report the generation of mice with a targeted disruption of the Socs5 gene. Socs5(-/-) mice were born in a normal Mendelian ratio and were healthy and fertile. We found that SOCS5 is expressed in primary B and T cells in wild-type mice. However, no abnormalities in the lymphocyte compartment were seen in SOCS5-deficient mice. We examined antigen- and cytokine-induced proliferative responses in B and T cells in the absence of SOCS5 and found no deviations from the responses seen in wild-type cells. Because SOCS5 has been implicated in Th1 differentiation, we also investigated the importance of SOCS5 in T helper cell responses. Unexpectedly, SOCS5-deficient CD4 T cells showed no abnormalities in Th1/Th2 differentiation and Socs5(-/-) mice showed normal resistance to infection with Leishmania major. Therefore, although SOCS5 is expressed in primary B and T cells, it appears to be dispensable for the regulation of lymphocyte function.
U2 - 10.1128/MCB.24.13.6094-6103.2004
DO - 10.1128/MCB.24.13.6094-6103.2004
M3 - Journal article
C2 - 15199163
VL - 24
SP - 6094
EP - 6103
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 13
ER -
ID: 10617056