TY - JOUR

T1 - Seroprevalence of SARS-CoV-2 Antibodies in Denmark

T2 - Results of Two Nationwide Population-Based Surveys, February and May 2021

AU - Krogsgaard, Lene Wulff

AU - Espenhain, Laura

AU - Tribler, Siri

AU - Jørgensen, Charlotte Sværke

AU - Hansen, Christian Holm

AU - Møller, Frederik Trier

AU - Helmuth, Ida Glode

AU - Sönksen, Ute Wolff

AU - Vangsted, Anne Marie

AU - Ullum, Henrik

AU - Ethelberg, Steen

N1 - Publisher Copyright: © 2023 Krogsgaard et al.

PY - 2023

Y1 - 2023

N2 - Background: Seroprevalence studies can be used to measure the progression of national COVID-19 epidemics. The Danish National Seroprevalence Survey of SARS-CoV-2 infections (DSS) was conducted as five separate surveys between May 2020 and May 2021. Here, we present results from the two last surveys conducted in February and May 2021. Methods: Persons aged 12 or older were randomly selected from the Danish Population Register and those having received COVID-19 vaccination subsequently excluded. Invitations to have blood drawn in local test centers were sent by mail. Samples were analyzed for whole Immunoglobulin by ELISA. Seroprevalence was estimated by sex, age and geography. Comparisons to vaccination uptake and RT-PCR test results were made. Results: In February 2021, we found detectable antibodies in 7.2% (95% CI: 6.3–7.9%) of the invited participants (participation rate 25%) and in May 2021 in 8.6% (95% CI: 7.6–9.5%) of the invited (participation rate: 14%). Seroprevalence did not differ by sex, but by age group, generally being higher among the <50 than 50+ year-olds. In May 2021, levels of seroprevalence varied from an estimated 13% (95% CI: 12–15%) in the capital to 5.2% (95% CI: 3.4–7.4%) in rural areas. Combining seroprevalence results with vaccine coverage, estimates of protection against infection in May 2021 varied from 95% among 65+ year-olds down to 10–20% among 12–40 year-olds. In March–May 2021, an estimated 80% of all community SARS-CoV-2 infections were diagnosed by RT-PCR and captured by surveillance. Conclusion: Seroprevalence estimates doubled during the 2020–21 winter wave of SARS-CoV-2 infections and then stabilized as vaccinations were rolled out. The epidemic affected large cities and younger people the most. Denmark saw comparatively low infections rates, but high test coverage; an estimated four out of five infections were detected by RT-PCR in March–May 2021.

AB - Background: Seroprevalence studies can be used to measure the progression of national COVID-19 epidemics. The Danish National Seroprevalence Survey of SARS-CoV-2 infections (DSS) was conducted as five separate surveys between May 2020 and May 2021. Here, we present results from the two last surveys conducted in February and May 2021. Methods: Persons aged 12 or older were randomly selected from the Danish Population Register and those having received COVID-19 vaccination subsequently excluded. Invitations to have blood drawn in local test centers were sent by mail. Samples were analyzed for whole Immunoglobulin by ELISA. Seroprevalence was estimated by sex, age and geography. Comparisons to vaccination uptake and RT-PCR test results were made. Results: In February 2021, we found detectable antibodies in 7.2% (95% CI: 6.3–7.9%) of the invited participants (participation rate 25%) and in May 2021 in 8.6% (95% CI: 7.6–9.5%) of the invited (participation rate: 14%). Seroprevalence did not differ by sex, but by age group, generally being higher among the <50 than 50+ year-olds. In May 2021, levels of seroprevalence varied from an estimated 13% (95% CI: 12–15%) in the capital to 5.2% (95% CI: 3.4–7.4%) in rural areas. Combining seroprevalence results with vaccine coverage, estimates of protection against infection in May 2021 varied from 95% among 65+ year-olds down to 10–20% among 12–40 year-olds. In March–May 2021, an estimated 80% of all community SARS-CoV-2 infections were diagnosed by RT-PCR and captured by surveillance. Conclusion: Seroprevalence estimates doubled during the 2020–21 winter wave of SARS-CoV-2 infections and then stabilized as vaccinations were rolled out. The epidemic affected large cities and younger people the most. Denmark saw comparatively low infections rates, but high test coverage; an estimated four out of five infections were detected by RT-PCR in March–May 2021.

KW - COVID-19 serological testing

KW - ELISA

KW - population register

KW - questionnaire

KW - SARS-CoV-2

KW - seroepidemiological studies

U2 - 10.2147/IDR.S383491

DO - 10.2147/IDR.S383491

M3 - Journal article

C2 - 36683911

AN - SCOPUS:85146528288

VL - 16

SP - 301

EP - 312

JO - Infection and Drug Resistance

JF - Infection and Drug Resistance

SN - 1178-6973

ER -