Selective three-phase liquid phase microextraction of acidic compounds from foodstuff simulants
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Selective three-phase liquid phase microextraction of acidic compounds from foodstuff simulants. / Rodríguez, A.; Pedersen-Bjergaard, S.; Rasmussen, K. E.; Nerín, C.
In: Journal of Chromatography A, Vol. 1198-1199, No. 1-2, 11.07.2008, p. 38-44.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Selective three-phase liquid phase microextraction of acidic compounds from foodstuff simulants
AU - Rodríguez, A.
AU - Pedersen-Bjergaard, S.
AU - Rasmussen, K. E.
AU - Nerín, C.
PY - 2008/7/11
Y1 - 2008/7/11
N2 - A three-phase liquid phase microextraction (LPME) technique with high selectivity for five aromatic carboxylic acids and three phenolic compounds has been developed and optimized. The system consists of an acidified donor phase, a thin layer of solvent inside the wall pores of a hollow fiber, and an alkaline acceptor phase located inside the hollow fiber. The analysis of the compounds in the acceptor phase was carried out by capillary electrophoresis with UV detection. Eugenol, thymol, and carvacrol were efficiently extracted from the aqueous solution using chloropentane as organic phase, with recoveries from 73.8% to 93.8%. However, using 2-octanone as the organic phase, the recoveries for the aromatic carboxylic acid compounds ranged from 60.7% to 93.7% whereas the phenols were not extracted. 2,6-naphthalene-dicarboxylic acid was found to remain in the organic phase. The influence of 10% ethanol and 3% acetic acid in the donor phase was deeply studied as these solutions are commonly used as food simulants. AS4 silicone oil was found to be the best organic phase for the extraction of phenols both in 3% acetic acid and matrices with a high content of alcohol. The results obtained are shown and discussed.
AB - A three-phase liquid phase microextraction (LPME) technique with high selectivity for five aromatic carboxylic acids and three phenolic compounds has been developed and optimized. The system consists of an acidified donor phase, a thin layer of solvent inside the wall pores of a hollow fiber, and an alkaline acceptor phase located inside the hollow fiber. The analysis of the compounds in the acceptor phase was carried out by capillary electrophoresis with UV detection. Eugenol, thymol, and carvacrol were efficiently extracted from the aqueous solution using chloropentane as organic phase, with recoveries from 73.8% to 93.8%. However, using 2-octanone as the organic phase, the recoveries for the aromatic carboxylic acid compounds ranged from 60.7% to 93.7% whereas the phenols were not extracted. 2,6-naphthalene-dicarboxylic acid was found to remain in the organic phase. The influence of 10% ethanol and 3% acetic acid in the donor phase was deeply studied as these solutions are commonly used as food simulants. AS4 silicone oil was found to be the best organic phase for the extraction of phenols both in 3% acetic acid and matrices with a high content of alcohol. The results obtained are shown and discussed.
KW - Capillary electrophoresis
KW - Food simulants
KW - Hollow fiber
KW - Liquid phase microextraction
KW - Phenols
KW - Specific migration analysis
UR - http://www.scopus.com/inward/record.url?scp=49849098682&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2008.05.032
DO - 10.1016/j.chroma.2008.05.032
M3 - Journal article
C2 - 18547581
AN - SCOPUS:49849098682
VL - 1198-1199
SP - 38
EP - 44
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0301-4770
IS - 1-2
ER -
ID: 231650193