Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities
Research output: Contribution to journal › Review › Research › peer-review
Documents
- Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities
Final published version, 3.5 MB, PDF document
Antigen-specific T-cells are highly variable, spanning potent antiviral efficacy and damaging auto-reactivity. In virus infections, identifying the most efficacious responses is critical to vaccine design. However, current methods depend on indirect measures or on ex vivo expanded CTL clones. We here describe a novel application of cytotoxic saporin-conjugated tetramers to kill antigen-specific T-cells without significant off-target effects. The relative efficacy of distinct antiviral CD8+ T-cell specificity can be directly assessed via antigen-specific CD8+ T-cell depletion. The utility of these reagents is demonstrated here in identifying the CD8+ T-cell specificity most effective in preventing HIV progression in HIV-infected HLA-B*27-positive immune controllers.
Original language | English |
---|---|
Article number | e0184496 |
Journal | PLOS ONE |
Volume | 12 |
Issue number | 10 |
Number of pages | 13 |
ISSN | 1932-6203 |
DOIs | |
Publication status | Published - Oct 2017 |
Number of downloads are based on statistics from Google Scholar and www.ku.dk
ID: 186874013