Rational and combinatorial engineering of the glucan synthesizing enzyme amylosucrase

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Rational and combinatorial engineering of the glucan synthesizing enzyme amylosucrase. / Albenne, C.; Van Der Veen, B.A.; Potocki-Véronèse, G.; Joucla, G.; Skov, L.; Mirza, Osman Asghar; Gajhede, M.; Monsan, P.; Remaud-Simeon, M.

In: Biocatalysis and Biotransformation, Vol. 21, No. 4-5, 01.08.2003, p. 271-277.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Albenne, C, Van Der Veen, BA, Potocki-Véronèse, G, Joucla, G, Skov, L, Mirza, OA, Gajhede, M, Monsan, P & Remaud-Simeon, M 2003, 'Rational and combinatorial engineering of the glucan synthesizing enzyme amylosucrase', Biocatalysis and Biotransformation, vol. 21, no. 4-5, pp. 271-277. https://doi.org/10.1080/10242420310001618537

APA

Albenne, C., Van Der Veen, B. A., Potocki-Véronèse, G., Joucla, G., Skov, L., Mirza, O. A., Gajhede, M., Monsan, P., & Remaud-Simeon, M. (2003). Rational and combinatorial engineering of the glucan synthesizing enzyme amylosucrase. Biocatalysis and Biotransformation, 21(4-5), 271-277. https://doi.org/10.1080/10242420310001618537

Vancouver

Albenne C, Van Der Veen BA, Potocki-Véronèse G, Joucla G, Skov L, Mirza OA et al. Rational and combinatorial engineering of the glucan synthesizing enzyme amylosucrase. Biocatalysis and Biotransformation. 2003 Aug 1;21(4-5):271-277. https://doi.org/10.1080/10242420310001618537

Author

Albenne, C. ; Van Der Veen, B.A. ; Potocki-Véronèse, G. ; Joucla, G. ; Skov, L. ; Mirza, Osman Asghar ; Gajhede, M. ; Monsan, P. ; Remaud-Simeon, M. / Rational and combinatorial engineering of the glucan synthesizing enzyme amylosucrase. In: Biocatalysis and Biotransformation. 2003 ; Vol. 21, No. 4-5. pp. 271-277.

Bibtex

@article{8c540bc6c32b424fbcce459e79e9159a,
title = "Rational and combinatorial engineering of the glucan synthesizing enzyme amylosucrase",
abstract = "Rational engineering of amylosucrase required detailed investigations of the molecular basis of catalysis. Biochemical characterization of the enzyme coupled to structural analyses enabled the polymerization mechanism to be elucidated. This provided key information for successfully changing amylosucrase to a hydrolase or an improved polymerase using site-directed mutagenesis. In parallel, a combinatorial approach was developed to further improve the catalyst. The method, based on random mutagenesis and recombination of parent sequences, has generated libraries of variants. These were searched for improved polymer formation using a first step of selection followed by a screening test including a double detection method. Several mutants with desired properties have been isolated, their sequences revealed that they could not have been designed following a rational approach.",
author = "C. Albenne and {Van Der Veen}, B.A. and G. Potocki-V{\'e}ron{\`e}se and G. Joucla and L. Skov and Mirza, {Osman Asghar} and M. Gajhede and P. Monsan and M. Remaud-Simeon",
year = "2003",
month = aug,
day = "1",
doi = "10.1080/10242420310001618537",
language = "English",
volume = "21",
pages = "271--277",
journal = "Biocatalysis and Biotransformation",
issn = "1024-2422",
publisher = "Taylor & Francis",
number = "4-5",

}

RIS

TY - JOUR

T1 - Rational and combinatorial engineering of the glucan synthesizing enzyme amylosucrase

AU - Albenne, C.

AU - Van Der Veen, B.A.

AU - Potocki-Véronèse, G.

AU - Joucla, G.

AU - Skov, L.

AU - Mirza, Osman Asghar

AU - Gajhede, M.

AU - Monsan, P.

AU - Remaud-Simeon, M.

PY - 2003/8/1

Y1 - 2003/8/1

N2 - Rational engineering of amylosucrase required detailed investigations of the molecular basis of catalysis. Biochemical characterization of the enzyme coupled to structural analyses enabled the polymerization mechanism to be elucidated. This provided key information for successfully changing amylosucrase to a hydrolase or an improved polymerase using site-directed mutagenesis. In parallel, a combinatorial approach was developed to further improve the catalyst. The method, based on random mutagenesis and recombination of parent sequences, has generated libraries of variants. These were searched for improved polymer formation using a first step of selection followed by a screening test including a double detection method. Several mutants with desired properties have been isolated, their sequences revealed that they could not have been designed following a rational approach.

AB - Rational engineering of amylosucrase required detailed investigations of the molecular basis of catalysis. Biochemical characterization of the enzyme coupled to structural analyses enabled the polymerization mechanism to be elucidated. This provided key information for successfully changing amylosucrase to a hydrolase or an improved polymerase using site-directed mutagenesis. In parallel, a combinatorial approach was developed to further improve the catalyst. The method, based on random mutagenesis and recombination of parent sequences, has generated libraries of variants. These were searched for improved polymer formation using a first step of selection followed by a screening test including a double detection method. Several mutants with desired properties have been isolated, their sequences revealed that they could not have been designed following a rational approach.

UR - http://www.scopus.com/inward/record.url?scp=0346707258&partnerID=8YFLogxK

U2 - 10.1080/10242420310001618537

DO - 10.1080/10242420310001618537

M3 - Journal article

AN - SCOPUS:0346707258

VL - 21

SP - 271

EP - 277

JO - Biocatalysis and Biotransformation

JF - Biocatalysis and Biotransformation

SN - 1024-2422

IS - 4-5

ER -

ID: 45670807