Protocol to study molecule exchange between venous blood and CSF in live mice using transcranial bioluminescence imaging
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In bioluminescence imaging (BLI), the biochemical reaction between a substrate and enzyme triggers light emission upon convergence. Unlike fluorescence imaging, BLI does not require excitation. In this protocol, we utilize the high signal-to-background ratio of the reaction between luciferase and its substrate to study the exchange of molecules between blood and cerebrospinal fluid. We outline steps for skull window thinning, cisterna magna infusion, intravascular retro-orbital injection, and imaging. For complete details on the use and execution of this protocol, please refer to Møllgård et al. (2023).1
Original language | English |
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Article number | 102652 |
Journal | STAR Protocols |
Volume | 4 |
Issue number | 4 |
Number of pages | 18 |
ISSN | 2666-1667 |
DOIs | |
Publication status | Published - 2023 |
Bibliographical note
Publisher Copyright:
© 2023 The Author(s)
- Health Sciences, Microscopy, Neuroscience
Research areas
ID: 371926664