Proteomics in the Study of Liver Diseases
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Standard
Proteomics in the Study of Liver Diseases. / Niu, Lili; Geyer, Philipp E. ; Mann, Matthias.
The Human Gut-Liver-Axis in Health and Disease. ed. / Aleksander Krag; Torben Hansen. Springer, 2018. p. 165-193.Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - CHAP
T1 - Proteomics in the Study of Liver Diseases
AU - Niu, Lili
AU - Geyer, Philipp E.
AU - Mann, Matthias
PY - 2018
Y1 - 2018
N2 - In this chapter, we describe the workflow of mass spectrometry (MS)-based proteomics with a focus on shotgun proteomics. We illustrate how MS-based proteomics can be applied to study liver pathophysiology using protein expression profiling, characterization of post-translational modifications (PTMs) and protein-protein interactions (PPIs). The publications on serum or plasma proteomics in the study of liver diseases during the years 2012 to 2017 are reviewed. We analyze the proportions of studies with regard to different kinds of liver disease and different proteomics workflows applied. Remarkably, outdated proteomics techniques were still being used in recent years and even account for a large proportion of the reviewed literature. The effort spent in different liver diseases is largely skewed to hepatocellular carcinoma and hepatic viral infection while a relatively small proportion focused on non-alcoholic fatty liver disease (NAFLD), which is the most prevalent liver disease worldwide. Finally, we describe plasma proteome profiling, a novel approach for biomarker discovery studies, and how this applies to liver diseases.
AB - In this chapter, we describe the workflow of mass spectrometry (MS)-based proteomics with a focus on shotgun proteomics. We illustrate how MS-based proteomics can be applied to study liver pathophysiology using protein expression profiling, characterization of post-translational modifications (PTMs) and protein-protein interactions (PPIs). The publications on serum or plasma proteomics in the study of liver diseases during the years 2012 to 2017 are reviewed. We analyze the proportions of studies with regard to different kinds of liver disease and different proteomics workflows applied. Remarkably, outdated proteomics techniques were still being used in recent years and even account for a large proportion of the reviewed literature. The effort spent in different liver diseases is largely skewed to hepatocellular carcinoma and hepatic viral infection while a relatively small proportion focused on non-alcoholic fatty liver disease (NAFLD), which is the most prevalent liver disease worldwide. Finally, we describe plasma proteome profiling, a novel approach for biomarker discovery studies, and how this applies to liver diseases.
U2 - 10.1007/978-3-319-98890-0_11
DO - 10.1007/978-3-319-98890-0_11
M3 - Book chapter
SN - 978-3-319-98889-4
SP - 165
EP - 193
BT - The Human Gut-Liver-Axis in Health and Disease
A2 - Krag, Aleksander
A2 - Hansen, Torben
PB - Springer
ER -
ID: 210006203