Production of anti-PfEMP1 polyclonal antisera in rats and mice
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Production of anti-PfEMP1 polyclonal antisera in rats and mice. / Olsen, Rebecca W; Suurbaar, Jennifer; Jensen, Anja Ramstedt.
Malaria Immunology: Targeting the Surface of Infected Erythrocytes. ed. / Anja Tatiana Ramstedt Jensen; Lars Hviid. Vol. 2470 Humana Press, 2022. p. 381-389 (Methods in molecular biology (Clifton, N.J.)).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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TY - CHAP
T1 - Production of anti-PfEMP1 polyclonal antisera in rats and mice
AU - Olsen, Rebecca W
AU - Suurbaar, Jennifer
AU - Jensen, Anja Ramstedt
N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Plasmodium falciparum-infected erythrocytes (IEs) bind various host receptors via members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family expressed on the surface of the IEs. Antibody reagents are needed to investigate interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells. This protocol describes the production of rat and mouse polyclonal anti-PfEMP1 antibodies. Polyclonal antibodies are relatively easy to produce and have advantages compared to monoclonal antibodies (see Chapters 28 - 30 ) for some applications. An ELISA-based method to test the polyclonal antibodies before their use in more advanced procedures is also presented.
AB - Plasmodium falciparum-infected erythrocytes (IEs) bind various host receptors via members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family expressed on the surface of the IEs. Antibody reagents are needed to investigate interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells. This protocol describes the production of rat and mouse polyclonal anti-PfEMP1 antibodies. Polyclonal antibodies are relatively easy to produce and have advantages compared to monoclonal antibodies (see Chapters 28 - 30 ) for some applications. An ELISA-based method to test the polyclonal antibodies before their use in more advanced procedures is also presented.
KW - Animals
KW - Antibodies, Protozoan
KW - Endothelial Cells/metabolism
KW - Erythrocytes/metabolism
KW - Humans
KW - Immune Sera/metabolism
KW - Malaria, Falciparum
KW - Mice
KW - Plasmodium falciparum/metabolism
KW - Protozoan Proteins/metabolism
KW - Rats
U2 - 10.1007/978-1-0716-2189-9_28
DO - 10.1007/978-1-0716-2189-9_28
M3 - Book chapter
C2 - 35881360
VL - 2470
T3 - Methods in molecular biology (Clifton, N.J.)
SP - 381
EP - 389
BT - Malaria Immunology
A2 - Jensen, Anja Tatiana Ramstedt
A2 - Hviid, Lars
PB - Humana Press
ER -
ID: 327466186