Preserved capacity for satellite cell proliferation, regeneration, and hypertrophy in the skeletal muscle of healthy elderly men

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Preserved capacity for satellite cell proliferation, regeneration, and hypertrophy in the skeletal muscle of healthy elderly men. / Karlsen, Anders; Soendenbroe, Casper ; Malmgaard‐clausen, Nikolaj M.; Wagener, Frederik; Moeller, Casper Emil; Senhaji, Zouhir; Damberg, Kristine; Andersen, Jesper Løvind; Schjerling, Peter; Kjaer, Michael; Mackey, Abigail L.

In: The FASEB Journal, Vol. 34, No. 5, 2020, p. 6418-6436.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Karlsen, A, Soendenbroe, C, Malmgaard‐clausen, NM, Wagener, F, Moeller, CE, Senhaji, Z, Damberg, K, Andersen, JL, Schjerling, P, Kjaer, M & Mackey, AL 2020, 'Preserved capacity for satellite cell proliferation, regeneration, and hypertrophy in the skeletal muscle of healthy elderly men', The FASEB Journal, vol. 34, no. 5, pp. 6418-6436. https://doi.org/10.1096/fj.202000196R

APA

Karlsen, A., Soendenbroe, C., Malmgaard‐clausen, N. M., Wagener, F., Moeller, C. E., Senhaji, Z., Damberg, K., Andersen, J. L., Schjerling, P., Kjaer, M., & Mackey, A. L. (2020). Preserved capacity for satellite cell proliferation, regeneration, and hypertrophy in the skeletal muscle of healthy elderly men. The FASEB Journal, 34(5), 6418-6436. https://doi.org/10.1096/fj.202000196R

Vancouver

Karlsen A, Soendenbroe C, Malmgaard‐clausen NM, Wagener F, Moeller CE, Senhaji Z et al. Preserved capacity for satellite cell proliferation, regeneration, and hypertrophy in the skeletal muscle of healthy elderly men. The FASEB Journal. 2020;34(5):6418-6436. https://doi.org/10.1096/fj.202000196R

Author

Karlsen, Anders ; Soendenbroe, Casper ; Malmgaard‐clausen, Nikolaj M. ; Wagener, Frederik ; Moeller, Casper Emil ; Senhaji, Zouhir ; Damberg, Kristine ; Andersen, Jesper Løvind ; Schjerling, Peter ; Kjaer, Michael ; Mackey, Abigail L. / Preserved capacity for satellite cell proliferation, regeneration, and hypertrophy in the skeletal muscle of healthy elderly men. In: The FASEB Journal. 2020 ; Vol. 34, No. 5. pp. 6418-6436.

Bibtex

@article{3948d8db9e9c4f1e86ca6e11b40f67d1,
title = "Preserved capacity for satellite cell proliferation, regeneration, and hypertrophy in the skeletal muscle of healthy elderly men",
abstract = "Blunted muscle hypertrophy and impaired regeneration with aging have been partly attributed to satellite cell (SC) dysfunction. However, true muscle regeneration has not yet been studied in elderly individuals. To investigate this, muscle injury was induced by 200 electrically stimulated (ES) eccentric contractions of the vastus lateralis (VL) of one leg in seven young (20-31 years) and 19 elderly men (60-73 years). This was followed by 13 weeks of resistance training (RT) for both legs to investigate the capacity for hypertrophy. Muscle biopsies were collected Pre- and Post-RT, and 9 days after ES, for immunohistochemistry and RT-PCR. Hypertrophy was assessed by MRI, DEXA, and immunohistochemistry. Overall, surprisingly comparable responses were observed between the young and elderly. Nine days after ES, Pax7+ SC number had doubled (P < .05), alongside necrosis and substantial changes in expression of genes related to matrix, myogenesis, and innervation (P < .05). Post-RT, VL cross-sectional area had increased in both legs (15%, P < .05) and SCs/type II fiber had increased 2-4 times more with ES+RT vs RT alone (P < .001). Together these novel findings demonstrate {"}youthful{"} regeneration and hypertrophy responses in human elderly muscle. Furthermore, boosting SC availability in healthy elderly men does not enhance the subsequent muscle hypertrophy response to RT.",
author = "Anders Karlsen and Casper Soendenbroe and Malmgaard‐clausen, {Nikolaj M.} and Frederik Wagener and Moeller, {Casper Emil} and Zouhir Senhaji and Kristine Damberg and Andersen, {Jesper L{\o}vind} and Peter Schjerling and Michael Kjaer and Mackey, {Abigail L.}",
year = "2020",
doi = "10.1096/fj.202000196R",
language = "English",
volume = "34",
pages = "6418--6436",
journal = "F A S E B Journal",
issn = "0892-6638",
publisher = "Federation of American Societies for Experimental Biology",
number = "5",

}

RIS

TY - JOUR

T1 - Preserved capacity for satellite cell proliferation, regeneration, and hypertrophy in the skeletal muscle of healthy elderly men

AU - Karlsen, Anders

AU - Soendenbroe, Casper

AU - Malmgaard‐clausen, Nikolaj M.

AU - Wagener, Frederik

AU - Moeller, Casper Emil

AU - Senhaji, Zouhir

AU - Damberg, Kristine

AU - Andersen, Jesper Løvind

AU - Schjerling, Peter

AU - Kjaer, Michael

AU - Mackey, Abigail L.

PY - 2020

Y1 - 2020

N2 - Blunted muscle hypertrophy and impaired regeneration with aging have been partly attributed to satellite cell (SC) dysfunction. However, true muscle regeneration has not yet been studied in elderly individuals. To investigate this, muscle injury was induced by 200 electrically stimulated (ES) eccentric contractions of the vastus lateralis (VL) of one leg in seven young (20-31 years) and 19 elderly men (60-73 years). This was followed by 13 weeks of resistance training (RT) for both legs to investigate the capacity for hypertrophy. Muscle biopsies were collected Pre- and Post-RT, and 9 days after ES, for immunohistochemistry and RT-PCR. Hypertrophy was assessed by MRI, DEXA, and immunohistochemistry. Overall, surprisingly comparable responses were observed between the young and elderly. Nine days after ES, Pax7+ SC number had doubled (P < .05), alongside necrosis and substantial changes in expression of genes related to matrix, myogenesis, and innervation (P < .05). Post-RT, VL cross-sectional area had increased in both legs (15%, P < .05) and SCs/type II fiber had increased 2-4 times more with ES+RT vs RT alone (P < .001). Together these novel findings demonstrate "youthful" regeneration and hypertrophy responses in human elderly muscle. Furthermore, boosting SC availability in healthy elderly men does not enhance the subsequent muscle hypertrophy response to RT.

AB - Blunted muscle hypertrophy and impaired regeneration with aging have been partly attributed to satellite cell (SC) dysfunction. However, true muscle regeneration has not yet been studied in elderly individuals. To investigate this, muscle injury was induced by 200 electrically stimulated (ES) eccentric contractions of the vastus lateralis (VL) of one leg in seven young (20-31 years) and 19 elderly men (60-73 years). This was followed by 13 weeks of resistance training (RT) for both legs to investigate the capacity for hypertrophy. Muscle biopsies were collected Pre- and Post-RT, and 9 days after ES, for immunohistochemistry and RT-PCR. Hypertrophy was assessed by MRI, DEXA, and immunohistochemistry. Overall, surprisingly comparable responses were observed between the young and elderly. Nine days after ES, Pax7+ SC number had doubled (P < .05), alongside necrosis and substantial changes in expression of genes related to matrix, myogenesis, and innervation (P < .05). Post-RT, VL cross-sectional area had increased in both legs (15%, P < .05) and SCs/type II fiber had increased 2-4 times more with ES+RT vs RT alone (P < .001). Together these novel findings demonstrate "youthful" regeneration and hypertrophy responses in human elderly muscle. Furthermore, boosting SC availability in healthy elderly men does not enhance the subsequent muscle hypertrophy response to RT.

U2 - 10.1096/fj.202000196R

DO - 10.1096/fj.202000196R

M3 - Journal article

C2 - 32167202

VL - 34

SP - 6418

EP - 6436

JO - F A S E B Journal

JF - F A S E B Journal

SN - 0892-6638

IS - 5

ER -

ID: 237525036