PR-DUB maintains expression of critical genes through FOXK1/2 and ASXL1/2/3-dependent recruitment to chromatin and H2AK119ub1 deubiquitination

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

PR-DUB maintains expression of critical genes through FOXK1/2 and ASXL1/2/3-dependent recruitment to chromatin and H2AK119ub1 deubiquitination. / Kolovos, Petros; Nishimura, Koutarou; Sankar, Aditya; Sidoli, Simone; Cloos, Paul A; Helin, Kristian; Christensen, Jesper.

In: Genome Research, Vol. 30, No. 8, 08.2020, p. 1119-1130.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kolovos, P, Nishimura, K, Sankar, A, Sidoli, S, Cloos, PA, Helin, K & Christensen, J 2020, 'PR-DUB maintains expression of critical genes through FOXK1/2 and ASXL1/2/3-dependent recruitment to chromatin and H2AK119ub1 deubiquitination', Genome Research, vol. 30, no. 8, pp. 1119-1130. https://doi.org/10.1101/gr.261016.120

APA

Kolovos, P., Nishimura, K., Sankar, A., Sidoli, S., Cloos, P. A., Helin, K., & Christensen, J. (2020). PR-DUB maintains expression of critical genes through FOXK1/2 and ASXL1/2/3-dependent recruitment to chromatin and H2AK119ub1 deubiquitination. Genome Research, 30(8), 1119-1130. https://doi.org/10.1101/gr.261016.120

Vancouver

Kolovos P, Nishimura K, Sankar A, Sidoli S, Cloos PA, Helin K et al. PR-DUB maintains expression of critical genes through FOXK1/2 and ASXL1/2/3-dependent recruitment to chromatin and H2AK119ub1 deubiquitination. Genome Research. 2020 Aug;30(8):1119-1130. https://doi.org/10.1101/gr.261016.120

Author

Kolovos, Petros ; Nishimura, Koutarou ; Sankar, Aditya ; Sidoli, Simone ; Cloos, Paul A ; Helin, Kristian ; Christensen, Jesper. / PR-DUB maintains expression of critical genes through FOXK1/2 and ASXL1/2/3-dependent recruitment to chromatin and H2AK119ub1 deubiquitination. In: Genome Research. 2020 ; Vol. 30, No. 8. pp. 1119-1130.

Bibtex

@article{38ebe688b30d46a9ae125bf5c6b2c654,
title = "PR-DUB maintains expression of critical genes through FOXK1/2 and ASXL1/2/3-dependent recruitment to chromatin and H2AK119ub1 deubiquitination",
abstract = "Polycomb group proteins are important for maintaining gene expression patterns and cell identity in metazoans. The mammalian Polycomb repressive de-ubiquitinase (PR-DUB) complexes catalyze removal of monoubiquitination on lysine 119 of histone H2A (H2AK119Ub1) through a multi-protein core comprised of BAP1, HCFC1, FOXK1/2, and OGT in combination with either of ASXL1, 2 or 3. Mutations in PR-DUB components are frequent in cancer. However, mechanistic understanding of PR-DUB function in gene regulation is limited. Here, we show that BAP1 is dependent on the ASXL proteins and FOXK1/2 in facilitating gene activation across the genome. Although PR-DUB previously was shown to cooperate with PRC2, we observed minimal overlap and functional interaction between BAP1 and PRC2 in embryonic stem cells. Collectively, these results demonstrate that PR-DUB, by counteracting accumulation of H2AK119ub1, maintains chromatin in an optimal configuration ensuring expression of genes important for general functions such as cell metabolism and homeostasis.",
author = "Petros Kolovos and Koutarou Nishimura and Aditya Sankar and Simone Sidoli and Cloos, {Paul A} and Kristian Helin and Jesper Christensen",
note = "Published by Cold Spring Harbor Laboratory Press.",
year = "2020",
month = aug,
doi = "10.1101/gr.261016.120",
language = "English",
volume = "30",
pages = "1119--1130",
journal = "Genome Research",
issn = "1088-9051",
publisher = "Cold Spring Harbor Laboratory Press",
number = "8",

}

RIS

TY - JOUR

T1 - PR-DUB maintains expression of critical genes through FOXK1/2 and ASXL1/2/3-dependent recruitment to chromatin and H2AK119ub1 deubiquitination

AU - Kolovos, Petros

AU - Nishimura, Koutarou

AU - Sankar, Aditya

AU - Sidoli, Simone

AU - Cloos, Paul A

AU - Helin, Kristian

AU - Christensen, Jesper

N1 - Published by Cold Spring Harbor Laboratory Press.

PY - 2020/8

Y1 - 2020/8

N2 - Polycomb group proteins are important for maintaining gene expression patterns and cell identity in metazoans. The mammalian Polycomb repressive de-ubiquitinase (PR-DUB) complexes catalyze removal of monoubiquitination on lysine 119 of histone H2A (H2AK119Ub1) through a multi-protein core comprised of BAP1, HCFC1, FOXK1/2, and OGT in combination with either of ASXL1, 2 or 3. Mutations in PR-DUB components are frequent in cancer. However, mechanistic understanding of PR-DUB function in gene regulation is limited. Here, we show that BAP1 is dependent on the ASXL proteins and FOXK1/2 in facilitating gene activation across the genome. Although PR-DUB previously was shown to cooperate with PRC2, we observed minimal overlap and functional interaction between BAP1 and PRC2 in embryonic stem cells. Collectively, these results demonstrate that PR-DUB, by counteracting accumulation of H2AK119ub1, maintains chromatin in an optimal configuration ensuring expression of genes important for general functions such as cell metabolism and homeostasis.

AB - Polycomb group proteins are important for maintaining gene expression patterns and cell identity in metazoans. The mammalian Polycomb repressive de-ubiquitinase (PR-DUB) complexes catalyze removal of monoubiquitination on lysine 119 of histone H2A (H2AK119Ub1) through a multi-protein core comprised of BAP1, HCFC1, FOXK1/2, and OGT in combination with either of ASXL1, 2 or 3. Mutations in PR-DUB components are frequent in cancer. However, mechanistic understanding of PR-DUB function in gene regulation is limited. Here, we show that BAP1 is dependent on the ASXL proteins and FOXK1/2 in facilitating gene activation across the genome. Although PR-DUB previously was shown to cooperate with PRC2, we observed minimal overlap and functional interaction between BAP1 and PRC2 in embryonic stem cells. Collectively, these results demonstrate that PR-DUB, by counteracting accumulation of H2AK119ub1, maintains chromatin in an optimal configuration ensuring expression of genes important for general functions such as cell metabolism and homeostasis.

U2 - 10.1101/gr.261016.120

DO - 10.1101/gr.261016.120

M3 - Journal article

C2 - 32747411

VL - 30

SP - 1119

EP - 1130

JO - Genome Research

JF - Genome Research

SN - 1088-9051

IS - 8

ER -

ID: 245895110