Polyamines preferentially interact with bent adenine tracts in double-stranded DNA
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Polyamines preferentially interact with bent adenine tracts in double-stranded DNA. / Lindemose, Søren; Nielsen, Peter E.; Møllegaard, Niels Erik.
In: Nucleic Acids Research, Vol. 33, No. 6, 2005, p. 1790-803.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Polyamines preferentially interact with bent adenine tracts in double-stranded DNA
AU - Lindemose, Søren
AU - Nielsen, Peter E.
AU - Møllegaard, Niels Erik
PY - 2005
Y1 - 2005
N2 - Polyamines, such as putrescine, spermidine and spermine, have indirectly been linked with the regulation of gene expression, and their concentrations are typically increased in cancer cells. Although effects on transcription factor binding to cognate DNA targets have been demonstrated, the mechanisms of the biological action of polyamines is poorly understood. Employing uranyl photo-probing we now demonstrate that polyamines at submillimolar concentrations bind preferentially to bent adenine tracts in double-stranded DNA. These results provide the first clear evidence for the sequence-specific binding of polyamines to DNA, and thereby suggest a mechanism by which the cellular effects of polyamines in terms of differential gene transcriptional activity could, at least partly, be a direct consequence of sequence-specific interactions of polyamines with promoters at the DNA sequence level.
AB - Polyamines, such as putrescine, spermidine and spermine, have indirectly been linked with the regulation of gene expression, and their concentrations are typically increased in cancer cells. Although effects on transcription factor binding to cognate DNA targets have been demonstrated, the mechanisms of the biological action of polyamines is poorly understood. Employing uranyl photo-probing we now demonstrate that polyamines at submillimolar concentrations bind preferentially to bent adenine tracts in double-stranded DNA. These results provide the first clear evidence for the sequence-specific binding of polyamines to DNA, and thereby suggest a mechanism by which the cellular effects of polyamines in terms of differential gene transcriptional activity could, at least partly, be a direct consequence of sequence-specific interactions of polyamines with promoters at the DNA sequence level.
KW - Adenine/metabolism
KW - Binding Sites
KW - DNA/chemistry
KW - Humans
KW - Nucleic Acid Conformation
KW - Polyamines/metabolism
KW - Promoter Regions, Genetic
KW - Uranyl Nitrate/chemistry
U2 - 10.1093/nar/gki319
DO - 10.1093/nar/gki319
M3 - Journal article
C2 - 15788751
VL - 33
SP - 1790
EP - 1803
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 6
ER -
ID: 203634944