Physical and transcript map of the region between D6S264 and D6S149 on chromosome 6q27, the minimal region of allele loss in sporadic epithelial ovarian cancer

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Standard

Physical and transcript map of the region between D6S264 and D6S149 on chromosome 6q27, the minimal region of allele loss in sporadic epithelial ovarian cancer. / Liu, Ying; Emilion, Gracy; Mungall, Andrew J; Dunham, Ian; Beck, Stephan; Le Meuth-Metzinger, Valerie G; Shelling, Andrew N; Charnock, Francis M L; Ganesan, Trivadi S.

In: Oncogene, Vol. 21, No. 3, 17.01.2002, p. 387-99.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Liu, Y, Emilion, G, Mungall, AJ, Dunham, I, Beck, S, Le Meuth-Metzinger, VG, Shelling, AN, Charnock, FML & Ganesan, TS 2002, 'Physical and transcript map of the region between D6S264 and D6S149 on chromosome 6q27, the minimal region of allele loss in sporadic epithelial ovarian cancer', Oncogene, vol. 21, no. 3, pp. 387-99. https://doi.org/10.1038/sj.onc.1205067

APA

Liu, Y., Emilion, G., Mungall, A. J., Dunham, I., Beck, S., Le Meuth-Metzinger, V. G., Shelling, A. N., Charnock, F. M. L., & Ganesan, T. S. (2002). Physical and transcript map of the region between D6S264 and D6S149 on chromosome 6q27, the minimal region of allele loss in sporadic epithelial ovarian cancer. Oncogene, 21(3), 387-99. https://doi.org/10.1038/sj.onc.1205067

Vancouver

Liu Y, Emilion G, Mungall AJ, Dunham I, Beck S, Le Meuth-Metzinger VG et al. Physical and transcript map of the region between D6S264 and D6S149 on chromosome 6q27, the minimal region of allele loss in sporadic epithelial ovarian cancer. Oncogene. 2002 Jan 17;21(3):387-99. https://doi.org/10.1038/sj.onc.1205067

Author

Liu, Ying ; Emilion, Gracy ; Mungall, Andrew J ; Dunham, Ian ; Beck, Stephan ; Le Meuth-Metzinger, Valerie G ; Shelling, Andrew N ; Charnock, Francis M L ; Ganesan, Trivadi S. / Physical and transcript map of the region between D6S264 and D6S149 on chromosome 6q27, the minimal region of allele loss in sporadic epithelial ovarian cancer. In: Oncogene. 2002 ; Vol. 21, No. 3. pp. 387-99.

Bibtex

@article{549e8954ab5d4e4481a1a79576410e46,
title = "Physical and transcript map of the region between D6S264 and D6S149 on chromosome 6q27, the minimal region of allele loss in sporadic epithelial ovarian cancer",
abstract = "We have previously shown a high frequency of allele loss at D6S193 (62%) on chromosomal arm 6q27 in ovarian tumours and mapped the minimal region of allele loss between D6S297 and D6S264 (3 cM). We isolated and mapped a single non-chimaeric YAC (17IA12, 260-280 kb) containing D6S193 and D6S297. A further extended bacterial contig (between D6S264 and D6S149) has been established using PACs and BACs and a transcript map has been established. We have mapped six new markers to the YAC; three of them are ESTs (WI-15078, WI-8751, and TCP10). We have isolated three cDNA clones of EST WI-15078 and one clone contains a complete open reading frame. The sequence shows homology to a new member of the ribonuclease family. The other two clones are splice variants of this new gene. The gene is expressed ubiquitously in normal tissues. It is expressed in 4/8 ovarian cancer cell lines by Northern analysis. The gene encodes for a 40 kDa protein. Direct sequencing of the gene in all the eight ovarian cancer cell lines did not identify any mutations. Clonogenic assays were performed by transfecting the full-length gene in to ovarian cancer cell lines and no suppression of growth was observed.",
keywords = "Amino Acid Sequence, Animals, Base Sequence, COS Cells, Chromosomes, Human, Pair 6, Colony-Forming Units Assay, Epithelial Cells, Exons, Expressed Sequence Tags, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Genes, Tumor Suppressor, Genetic Markers, Humans, Introns, Loss of Heterozygosity, Molecular Sequence Data, Ovarian Neoplasms, Physical Chromosome Mapping, RNA, Messenger, Ribonucleases, Sequence Alignment, Transcription, Genetic, Tumor Cells, Cultured",
author = "Ying Liu and Gracy Emilion and Mungall, {Andrew J} and Ian Dunham and Stephan Beck and {Le Meuth-Metzinger}, {Valerie G} and Shelling, {Andrew N} and Charnock, {Francis M L} and Ganesan, {Trivadi S}",
year = "2002",
month = jan,
day = "17",
doi = "10.1038/sj.onc.1205067",
language = "English",
volume = "21",
pages = "387--99",
journal = "Oncogene",
issn = "0950-9232",
publisher = "nature publishing group",
number = "3",

}

RIS

TY - JOUR

T1 - Physical and transcript map of the region between D6S264 and D6S149 on chromosome 6q27, the minimal region of allele loss in sporadic epithelial ovarian cancer

AU - Liu, Ying

AU - Emilion, Gracy

AU - Mungall, Andrew J

AU - Dunham, Ian

AU - Beck, Stephan

AU - Le Meuth-Metzinger, Valerie G

AU - Shelling, Andrew N

AU - Charnock, Francis M L

AU - Ganesan, Trivadi S

PY - 2002/1/17

Y1 - 2002/1/17

N2 - We have previously shown a high frequency of allele loss at D6S193 (62%) on chromosomal arm 6q27 in ovarian tumours and mapped the minimal region of allele loss between D6S297 and D6S264 (3 cM). We isolated and mapped a single non-chimaeric YAC (17IA12, 260-280 kb) containing D6S193 and D6S297. A further extended bacterial contig (between D6S264 and D6S149) has been established using PACs and BACs and a transcript map has been established. We have mapped six new markers to the YAC; three of them are ESTs (WI-15078, WI-8751, and TCP10). We have isolated three cDNA clones of EST WI-15078 and one clone contains a complete open reading frame. The sequence shows homology to a new member of the ribonuclease family. The other two clones are splice variants of this new gene. The gene is expressed ubiquitously in normal tissues. It is expressed in 4/8 ovarian cancer cell lines by Northern analysis. The gene encodes for a 40 kDa protein. Direct sequencing of the gene in all the eight ovarian cancer cell lines did not identify any mutations. Clonogenic assays were performed by transfecting the full-length gene in to ovarian cancer cell lines and no suppression of growth was observed.

AB - We have previously shown a high frequency of allele loss at D6S193 (62%) on chromosomal arm 6q27 in ovarian tumours and mapped the minimal region of allele loss between D6S297 and D6S264 (3 cM). We isolated and mapped a single non-chimaeric YAC (17IA12, 260-280 kb) containing D6S193 and D6S297. A further extended bacterial contig (between D6S264 and D6S149) has been established using PACs and BACs and a transcript map has been established. We have mapped six new markers to the YAC; three of them are ESTs (WI-15078, WI-8751, and TCP10). We have isolated three cDNA clones of EST WI-15078 and one clone contains a complete open reading frame. The sequence shows homology to a new member of the ribonuclease family. The other two clones are splice variants of this new gene. The gene is expressed ubiquitously in normal tissues. It is expressed in 4/8 ovarian cancer cell lines by Northern analysis. The gene encodes for a 40 kDa protein. Direct sequencing of the gene in all the eight ovarian cancer cell lines did not identify any mutations. Clonogenic assays were performed by transfecting the full-length gene in to ovarian cancer cell lines and no suppression of growth was observed.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - COS Cells

KW - Chromosomes, Human, Pair 6

KW - Colony-Forming Units Assay

KW - Epithelial Cells

KW - Exons

KW - Expressed Sequence Tags

KW - Female

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Neoplastic

KW - Genes, Tumor Suppressor

KW - Genetic Markers

KW - Humans

KW - Introns

KW - Loss of Heterozygosity

KW - Molecular Sequence Data

KW - Ovarian Neoplasms

KW - Physical Chromosome Mapping

KW - RNA, Messenger

KW - Ribonucleases

KW - Sequence Alignment

KW - Transcription, Genetic

KW - Tumor Cells, Cultured

U2 - 10.1038/sj.onc.1205067

DO - 10.1038/sj.onc.1205067

M3 - Journal article

C2 - 11821951

VL - 21

SP - 387

EP - 399

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 3

ER -

ID: 33572722