PCR typing of two short tandem repeat (STR) structures upstreams of the human myelin basic protein (MBP) gene: The genetic susceptibility in multiple sclerosis and monosymptomatic idiopathic optic neuritis in Danes
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PCR typing of two short tandem repeat (STR) structures upstreams of the human myelin basic protein (MBP) gene : The genetic susceptibility in multiple sclerosis and monosymptomatic idiopathic optic neuritis in Danes. / Nellemann, L J; Frederiksen, J; Morling, N.
In: Multiple Sclerosis, Vol. 1, No. 3, 1995, p. 186-9.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - PCR typing of two short tandem repeat (STR) structures upstreams of the human myelin basic protein (MBP) gene
T2 - The genetic susceptibility in multiple sclerosis and monosymptomatic idiopathic optic neuritis in Danes
AU - Nellemann, L J
AU - Frederiksen, J
AU - Morling, N
N1 - Keywords: Alleles; Chromosome Deletion; Cloning, Molecular; DNA; Denmark; Gene Frequency; Genes; Genetic Predisposition to Disease; Humans; Microsatellite Repeats; Multiple Sclerosis; Myelin Basic Proteins; Optic Neuritis; Polymerase Chain Reaction
PY - 1995
Y1 - 1995
N2 - We investigated two short tandem tetranucleotide (TGGA) repeat polymorphisms upstreams of the myelin basic protein (MBP) gene. The region was amplified by the polymerase chain reaction (PCR) and the two repeat systems were separated by cutting with the restriction enzyme NlaIII. The lengths of the DNA fragments were analyzed by vertical electrophoresis in polyacrylamide gels followed by silver staining. We compared the DNA fragment frequencies of the two MBP regions in 34 patients suffering from multiple sclerosis and in 78 suffering from monosymptomatic idiopathic optic neuritis to those in 200 healthy controls. We found no significant differences between the MBP fragment frequencies in either of the patient groups and in the control group.
AB - We investigated two short tandem tetranucleotide (TGGA) repeat polymorphisms upstreams of the myelin basic protein (MBP) gene. The region was amplified by the polymerase chain reaction (PCR) and the two repeat systems were separated by cutting with the restriction enzyme NlaIII. The lengths of the DNA fragments were analyzed by vertical electrophoresis in polyacrylamide gels followed by silver staining. We compared the DNA fragment frequencies of the two MBP regions in 34 patients suffering from multiple sclerosis and in 78 suffering from monosymptomatic idiopathic optic neuritis to those in 200 healthy controls. We found no significant differences between the MBP fragment frequencies in either of the patient groups and in the control group.
M3 - Journal article
C2 - 9345452
VL - 1
SP - 186
EP - 189
JO - Multiple Sclerosis Journal
JF - Multiple Sclerosis Journal
SN - 1352-4585
IS - 3
ER -
ID: 16186051