Overproduction of PIB-Type ATPases
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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Overproduction of PIB-Type ATPases. / Liu, Xiangyu; Sitsel, Oleg; Wang, Kaituo; Gourdon, Pontus.
P-Type ATPases: Methods and Protocols. ed. / Maike Bublitz. Vol. 1377 Springer, 2016. p. 29-36 (Methods in Molecular Biology, Vol. 1377).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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TY - CHAP
T1 - Overproduction of PIB-Type ATPases
AU - Liu, Xiangyu
AU - Sitsel, Oleg
AU - Wang, Kaituo
AU - Gourdon, Pontus
PY - 2016
Y1 - 2016
N2 - Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals.
AB - Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals.
U2 - 10.1007/978-1-4939-3179-8_5
DO - 10.1007/978-1-4939-3179-8_5
M3 - Book chapter
C2 - 26695020
SN - 978-1-4939-3178-1
VL - 1377
T3 - Methods in Molecular Biology
SP - 29
EP - 36
BT - P-Type ATPases
A2 - Bublitz, Maike
PB - Springer
ER -
ID: 171590537