Optimization of rVAR2-based isolation of cancer cells in blood for building a robust assay for clinical detection of circulating tumor cells
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Optimization of rVAR2-based isolation of cancer cells in blood for building a robust assay for clinical detection of circulating tumor cells. / Sand, Nicolai T; Petersen, Tobias B; Bang-Christensen, Sara R; Ahrens, Theresa D; Løppke, Caroline; Jørgensen, Amalie M; Gustavsson, Tobias; Choudhary, Swati; Theander, Thor G; Salanti, Ali; Agerbæk, Mette Ø.
In: International Journal of Molecular Sciences , Vol. 21, No. 7, 2401, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Optimization of rVAR2-based isolation of cancer cells in blood for building a robust assay for clinical detection of circulating tumor cells
AU - Sand, Nicolai T
AU - Petersen, Tobias B
AU - Bang-Christensen, Sara R
AU - Ahrens, Theresa D
AU - Løppke, Caroline
AU - Jørgensen, Amalie M
AU - Gustavsson, Tobias
AU - Choudhary, Swati
AU - Theander, Thor G
AU - Salanti, Ali
AU - Agerbæk, Mette Ø
PY - 2020
Y1 - 2020
N2 - Early detection and monitoring of cancer progression is key to successful treatment. Therefore, much research is invested in developing technologies, enabling effective and valuable use of non-invasive liquid biopsies. This includes the detection and analysis of circulating tumor cells (CTCs) from blood samples. Recombinant malaria protein VAR2CSA (rVAR2) binds a unique chondroitin sulfate modification present on the vast majority of cancers and thereby holds promise as a near-universal tumor cell-targeting reagent to isolate CTCs from complex blood samples. This study describes a technical approach for optimizing the coupling of rVAR2 to magnetic beads and the development of a CTC isolation platform targeting a range of different cancer cell lines. We investigate both direct and indirect approaches for rVAR2-mediated bead retrieval of cancer cells and conclude that an indirect capture approach is most effective for rVAR2-based cancer cell retrieval.
AB - Early detection and monitoring of cancer progression is key to successful treatment. Therefore, much research is invested in developing technologies, enabling effective and valuable use of non-invasive liquid biopsies. This includes the detection and analysis of circulating tumor cells (CTCs) from blood samples. Recombinant malaria protein VAR2CSA (rVAR2) binds a unique chondroitin sulfate modification present on the vast majority of cancers and thereby holds promise as a near-universal tumor cell-targeting reagent to isolate CTCs from complex blood samples. This study describes a technical approach for optimizing the coupling of rVAR2 to magnetic beads and the development of a CTC isolation platform targeting a range of different cancer cell lines. We investigate both direct and indirect approaches for rVAR2-mediated bead retrieval of cancer cells and conclude that an indirect capture approach is most effective for rVAR2-based cancer cell retrieval.
U2 - 10.3390/ijms21072401
DO - 10.3390/ijms21072401
M3 - Journal article
C2 - 32244341
VL - 21
JO - International Journal of Molecular Sciences (Online)
JF - International Journal of Molecular Sciences (Online)
SN - 1661-6596
IS - 7
M1 - 2401
ER -
ID: 239176855