Optical investigation of intra- and extra-cellular dynamics

Research output: Book/Report › Ph.D. thesis › Research

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Optical investigation of intra- and extra-cellular dynamics. / Leijnse, Natascha.

The Niels Bohr Institute, Faculty of Science, University of Copenhagen, 2013. 132 p.

Research output: Book/Report › Ph.D. thesis › Research

Harvard

Leijnse, N 2013, Optical investigation of intra- and extra-cellular dynamics. The Niels Bohr Institute, Faculty of Science, University of Copenhagen. <https://soeg.kb.dk/permalink/45KBDK_KGL/fbp0ps/alma99122889237105763>

APA

Leijnse, N. (2013). Optical investigation of intra- and extra-cellular dynamics. The Niels Bohr Institute, Faculty of Science, University of Copenhagen. https://soeg.kb.dk/permalink/45KBDK_KGL/fbp0ps/alma99122889237105763

Vancouver

Leijnse N. Optical investigation of intra- and extra-cellular dynamics. The Niels Bohr Institute, Faculty of Science, University of Copenhagen, 2013. 132 p.

Author

Leijnse, Natascha. / Optical investigation of intra- and extra-cellular dynamics. The Niels Bohr Institute, Faculty of Science, University of Copenhagen, 2013. 132 p.

Bibtex

@phdthesis{3baf15ead618486199daea2ea2e9882d,
title = "Optical investigation of intra- and extra-cellular dynamics",
abstract = "In this thesis we investigated intra- and extra-cellular dynamics with the help of optical tweezers and confocal imaging.Firstly, we measured in vivo diffusion inside endothelial cells by tracking lipid granules and compared the results to in vitro diffusion of polystyrene beads in wormlike micelles.Furthermore, we studied F-actin dynamics in, and curvature sensing of, a receptor and its ligand on artificially pulled membrane tethers.Force measurements between a cell and an approaching optically trapped bead were conducted in order to probe the endothelial cell's glycocalyx.Finally, investigations of force generation by living cells embedded in 3D matrices, conducted in Alexander Dunn's lab at Stanford University, are described in the last chapter.",
author = "Natascha Leijnse",
note = "Thesis is not public.",
year = "2013",
language = "English",
publisher = "The Niels Bohr Institute, Faculty of Science, University of Copenhagen",

}

RIS

TY - BOOK

T1 - Optical investigation of intra- and extra-cellular dynamics

AU - Leijnse, Natascha

N1 - Thesis is not public.

PY - 2013

Y1 - 2013

N2 - In this thesis we investigated intra- and extra-cellular dynamics with the help of optical tweezers and confocal imaging.Firstly, we measured in vivo diffusion inside endothelial cells by tracking lipid granules and compared the results to in vitro diffusion of polystyrene beads in wormlike micelles.Furthermore, we studied F-actin dynamics in, and curvature sensing of, a receptor and its ligand on artificially pulled membrane tethers.Force measurements between a cell and an approaching optically trapped bead were conducted in order to probe the endothelial cell's glycocalyx.Finally, investigations of force generation by living cells embedded in 3D matrices, conducted in Alexander Dunn's lab at Stanford University, are described in the last chapter.

AB - In this thesis we investigated intra- and extra-cellular dynamics with the help of optical tweezers and confocal imaging.Firstly, we measured in vivo diffusion inside endothelial cells by tracking lipid granules and compared the results to in vitro diffusion of polystyrene beads in wormlike micelles.Furthermore, we studied F-actin dynamics in, and curvature sensing of, a receptor and its ligand on artificially pulled membrane tethers.Force measurements between a cell and an approaching optically trapped bead were conducted in order to probe the endothelial cell's glycocalyx.Finally, investigations of force generation by living cells embedded in 3D matrices, conducted in Alexander Dunn's lab at Stanford University, are described in the last chapter.

UR - https://soeg.kb.dk/permalink/45KBDK_KGL/fbp0ps/alma99122889237105763

M3 - Ph.D. thesis

BT - Optical investigation of intra- and extra-cellular dynamics

PB - The Niels Bohr Institute, Faculty of Science, University of Copenhagen

ER -

ID: 88025319