Optical and Hydrodynamic Stretching of Single Cells from Blood

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

  • Henrik Thirstrup
  • Tony B. Rungling
  • Mustafa Zyad Khalil Al-Hamdani
  • Ragavan Pathanchalinathan
  • Dziegiel, Morten Hanefeld
  • Anders Kristensen
  • Rodolphe Marie
  • Kirstine Berg-Sørensen

Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor. In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step. Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single "interesting" cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.

Original languageEnglish
Title of host publicationOptical Trapping Applications, OTA 2017
VolumePart F64-OTA 2017
PublisherThe Optical Society (OSA)
Publication date1 Jan 2017
Article numberOtM4E.1
ISBN (Electronic)9781557528209
Publication statusPublished - 1 Jan 2017
EventOptical Trapping Applications, OTA 2017 - San Diego, United States
Duration: 2 Apr 20175 Apr 2017


ConferenceOptical Trapping Applications, OTA 2017
LandUnited States
BySan Diego

ID: 198566327