Naïve human pluripotent stem cells respond to Wnt, Nodal and LIF signalling to produce expandable naïve extra-embryonic endoderm
Research output: Contribution to journal › Journal article › Research › peer-review
Documents
- dev180620
Final published version, 13.2 MB, PDF document
Embryonic stem cells (ESCs) exist in at least two states that transcriptionally resemble different stages of embryonic development. Naïve ESCs resemble peri-implantation stages and primed ESCs the pre-gastrulation epiblast. In mouse, primed ESCs give rise to definitive endoderm in response to the pathways downstream of Nodal and Wnt signalling. However, when these pathways are activated in naïve ESCs, they differentiate to a cell type resembling early primitive endoderm (PrE), the blastocyst-stage progenitor of the extra-embryonic endoderm. Here, we apply this context dependency to human ESCs, showing that activation of Nodal and Wnt signalling drives the differentiation of naïve pluripotent cells toward extra-embryonic PrE, or hypoblast, and these can be expanded as an in vitro model for naïve extra-embryonic endoderm (nEnd). Consistent with observations made in mouse, human PrE differentiation is dependent on FGF signalling in vitro, and we show that, by inhibiting FGF receptor signalling, we can simplify naïve pluripotent culture conditions, such that the inhibitor requirements closer resemble those used in mouse. The expandable nEnd cultures reported here represent stable extra-embryonic endoderm, or human hypoblast, cell lines.This article has an associated 'The people behind the papers' interview.
Original language | English |
---|---|
Article number | 180620 |
Journal | Development (Cambridge, England) |
Volume | 146 |
Issue number | 24 |
Number of pages | 15 |
ISSN | 0950-1991 |
DOIs | |
Publication status | Published - 16 Dec 2019 |
Bibliographical note
© 2019. Published by The Company of Biologists Ltd.
Number of downloads are based on statistics from Google Scholar and www.ku.dk
ID: 235344434