NAD+ augmentation restores mitophagy and limits accelerated aging in Werner syndrome

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Documents

  • Evandro F Fang
  • Yujun Hou
  • Sofie Lautrup
  • Martin Borch Jensen
  • Beimeng Yang
  • Tanima SenGupta
  • Domenica Caponio
  • Rojyar Khezri
  • Tyler G Demarest
  • Yahyah Aman
  • David Figueroa
  • Marya Morevati
  • Ho-Joon Lee
  • Hisaya Kato
  • Henok Kassahun
  • Jong-Hyuk Lee
  • Deborah Filippelli
  • Mustafa Nazir Okur
  • Aswin Mangerich
  • Deborah L Croteau
  • Yoshiro Maezawa
  • Costas A Lyssiotis
  • Jun Tao
  • Koutaro Yokote
  • Tor Erik Rusten
  • Mark P Mattson
  • Heinrich Jasper
  • Hilde Nilsen

Metabolic dysfunction is a primary feature of Werner syndrome (WS), a human premature aging disease caused by mutations in the gene encoding the Werner (WRN) DNA helicase. WS patients exhibit severe metabolic phenotypes, but the underlying mechanisms are not understood, and whether the metabolic deficit can be targeted for therapeutic intervention has not been determined. Here we report impaired mitophagy and depletion of NAD+, a fundamental ubiquitous molecule, in WS patient samples and WS invertebrate models. WRN regulates transcription of a key NAD+ biosynthetic enzyme nicotinamide nucleotide adenylyltransferase 1 (NMNAT1). NAD+ repletion restores NAD+ metabolic profiles and improves mitochondrial quality through DCT-1 and ULK-1-dependent mitophagy. At the organismal level, NAD+ repletion remarkably extends lifespan and delays accelerated aging, including stem cell dysfunction, in Caenorhabditis elegans and Drosophila melanogaster models of WS. Our findings suggest that accelerated aging in WS is mediated by impaired mitochondrial function and mitophagy, and that bolstering cellular NAD+ levels counteracts WS phenotypes.

Original languageEnglish
Article number5284
JournalNature Communications
Volume10
Issue number1
Number of pages18
ISSN2041-1723
DOIs
Publication statusPublished - 2019

ID: 236986472