Molecular evolution of GB virus B hepatitis virus during acute resolving and persistent infections in experimentally infected tamarins
Research output: Contribution to journal › Journal article › Research › peer-review
GB virus B (GBV-B) causes acute hepatitis in experimentally infected tamarins. We compared evolutionary features in acute resolving and persistent GBV-B infection. We detected no evidence of evolution in four animals with clearance during weeks 9-12, whereas three animals with clearance during weeks 13-26 had several substitutions in their polyprotein sequence. A single tamarin had long-term GBV-B viraemia; analysis of virus recovered at weeks 2, 5, 12, 20, 26, 52 and 104 demonstrated that mutations accumulated over time. Overall, the amino acid substitution rate was 3.5x10(-3) and 1.1x10(-3) substitutions per site year(-1) during weeks 1-52 and 53-104, respectively. Thus, there was a significant decrease in evolution over time, as found for hepatitis C virus. The rate of non-synonymous substitution per non-synonymous site compared with that of synonymous substitution per synonymous site decreased over time, suggesting reduction of positive selective pressure. These data demonstrate that prolonged GBV-B infection is associated with viral evolution.
Original language | English |
---|---|
Journal | Journal of General Virology |
Volume | 91 |
Issue number | Pt 3 |
Pages (from-to) | 727-33 |
Number of pages | 6 |
ISSN | 0022-1317 |
DOIs | |
Publication status | Published - 2010 |
Bibliographical note
Keywords: Amino Acid Substitution; Animals; Disease Models, Animal; Evolution, Molecular; GB virus B; Gene Products, pol; Hepatitis, Viral, Animal; Leontopithecus; Monkey Diseases
ID: 21331808