Modulation of the Lactobacillus acidophilus La-5 lipidome by different growth conditions
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Modulation of the Lactobacillus acidophilus La-5 lipidome by different growth conditions. / Hansen, Marie-Louise Rittermann W; Clausen, Anders; Ejsing, Christer S.; Risbo, Jens.
In: Microbiology, Vol. 161, No. 10, 2015, p. 1990-1998.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Modulation of the Lactobacillus acidophilus La-5 lipidome by different growth conditions
AU - Hansen, Marie-Louise Rittermann W
AU - Clausen, Anders
AU - Ejsing, Christer S.
AU - Risbo, Jens
PY - 2015
Y1 - 2015
N2 - Probiotics are bacteria used in the food industry due to their potential health benefits. In this study, the plasma membrane of the probiotic Lactobacillus acidophilus La-5 was investigated using state-of-the-art high-resolution shotgun lipidomics. Comparisons of the lipidome of the plasma membrane were done after altering the fatty acid composition by supplementing L. acidophilus La-5 with saturated, mono-, di- and tri-unsaturated fatty acids during fermentation. The plasma membrane with the highest degree of saturation resulted in a lipid composition with the highest proportion of cardiolipin (CL) and lowest proportion of monolysocardiolipin (MLCL). No significant changes were found for other lipid classes. The bacteria grown with di- and tri-unsaturated fatty acids were expected to have more unsaturated plasma membranes than bacteria grown with mono-unsaturated fatty acids. This was also the case for MLCL, but the numbers of double bonds for CL were quite similar for these three samples. The results indicate that L. acidophilus La-5 possesses a molecular mechanism for remodelling and optimizing the fatty acid composition of CL and MLCL species and the molar ratio of CL and MLCL. This study contributes new knowledge on the previously uninvestigated lipidome of L. acidophilus La-5.
AB - Probiotics are bacteria used in the food industry due to their potential health benefits. In this study, the plasma membrane of the probiotic Lactobacillus acidophilus La-5 was investigated using state-of-the-art high-resolution shotgun lipidomics. Comparisons of the lipidome of the plasma membrane were done after altering the fatty acid composition by supplementing L. acidophilus La-5 with saturated, mono-, di- and tri-unsaturated fatty acids during fermentation. The plasma membrane with the highest degree of saturation resulted in a lipid composition with the highest proportion of cardiolipin (CL) and lowest proportion of monolysocardiolipin (MLCL). No significant changes were found for other lipid classes. The bacteria grown with di- and tri-unsaturated fatty acids were expected to have more unsaturated plasma membranes than bacteria grown with mono-unsaturated fatty acids. This was also the case for MLCL, but the numbers of double bonds for CL were quite similar for these three samples. The results indicate that L. acidophilus La-5 possesses a molecular mechanism for remodelling and optimizing the fatty acid composition of CL and MLCL species and the molar ratio of CL and MLCL. This study contributes new knowledge on the previously uninvestigated lipidome of L. acidophilus La-5.
U2 - 10.1099/mic.0.000145
DO - 10.1099/mic.0.000145
M3 - Journal article
C2 - 26197785
VL - 161
SP - 1990
EP - 1998
JO - Microbiology
JF - Microbiology
SN - 1350-0872
IS - 10
ER -
ID: 147626220