Modulation of the Lactobacillus acidophilus La-5 lipidome by different growth conditions

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Modulation of the Lactobacillus acidophilus La-5 lipidome by different growth conditions. / Hansen, Marie-Louise Rittermann W; Clausen, Anders; Ejsing, Christer S.; Risbo, Jens.

In: Microbiology, Vol. 161, No. 10, 2015, p. 1990-1998.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Hansen, M-LRW, Clausen, A, Ejsing, CS & Risbo, J 2015, 'Modulation of the Lactobacillus acidophilus La-5 lipidome by different growth conditions', Microbiology, vol. 161, no. 10, pp. 1990-1998. https://doi.org/10.1099/mic.0.000145

APA

Hansen, M-L. R. W., Clausen, A., Ejsing, C. S., & Risbo, J. (2015). Modulation of the Lactobacillus acidophilus La-5 lipidome by different growth conditions. Microbiology, 161(10), 1990-1998. https://doi.org/10.1099/mic.0.000145

Vancouver

Hansen M-LRW, Clausen A, Ejsing CS, Risbo J. Modulation of the Lactobacillus acidophilus La-5 lipidome by different growth conditions. Microbiology. 2015;161(10):1990-1998. https://doi.org/10.1099/mic.0.000145

Author

Hansen, Marie-Louise Rittermann W ; Clausen, Anders ; Ejsing, Christer S. ; Risbo, Jens. / Modulation of the Lactobacillus acidophilus La-5 lipidome by different growth conditions. In: Microbiology. 2015 ; Vol. 161, No. 10. pp. 1990-1998.

Bibtex

@article{89978472cf414846a82972b2f97d8672,
title = "Modulation of the Lactobacillus acidophilus La-5 lipidome by different growth conditions",
abstract = "Probiotics are bacteria used in the food industry due to their potential health benefits. In this study, the plasma membrane of the probiotic Lactobacillus acidophilus La-5 was investigated using state-of-the-art high-resolution shotgun lipidomics. Comparisons of the lipidome of the plasma membrane were done after altering the fatty acid composition by supplementing L. acidophilus La-5 with saturated, mono-, di- and tri-unsaturated fatty acids during fermentation. The plasma membrane with the highest degree of saturation resulted in a lipid composition with the highest proportion of cardiolipin (CL) and lowest proportion of monolysocardiolipin (MLCL). No significant changes were found for other lipid classes. The bacteria grown with di- and tri-unsaturated fatty acids were expected to have more unsaturated plasma membranes than bacteria grown with mono-unsaturated fatty acids. This was also the case for MLCL, but the numbers of double bonds for CL were quite similar for these three samples. The results indicate that L. acidophilus La-5 possesses a molecular mechanism for remodelling and optimizing the fatty acid composition of CL and MLCL species and the molar ratio of CL and MLCL. This study contributes new knowledge on the previously uninvestigated lipidome of L. acidophilus La-5.",
author = "Hansen, {Marie-Louise Rittermann W} and Anders Clausen and Ejsing, {Christer S.} and Jens Risbo",
year = "2015",
doi = "10.1099/mic.0.000145",
language = "English",
volume = "161",
pages = "1990--1998",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "10",

}

RIS

TY - JOUR

T1 - Modulation of the Lactobacillus acidophilus La-5 lipidome by different growth conditions

AU - Hansen, Marie-Louise Rittermann W

AU - Clausen, Anders

AU - Ejsing, Christer S.

AU - Risbo, Jens

PY - 2015

Y1 - 2015

N2 - Probiotics are bacteria used in the food industry due to their potential health benefits. In this study, the plasma membrane of the probiotic Lactobacillus acidophilus La-5 was investigated using state-of-the-art high-resolution shotgun lipidomics. Comparisons of the lipidome of the plasma membrane were done after altering the fatty acid composition by supplementing L. acidophilus La-5 with saturated, mono-, di- and tri-unsaturated fatty acids during fermentation. The plasma membrane with the highest degree of saturation resulted in a lipid composition with the highest proportion of cardiolipin (CL) and lowest proportion of monolysocardiolipin (MLCL). No significant changes were found for other lipid classes. The bacteria grown with di- and tri-unsaturated fatty acids were expected to have more unsaturated plasma membranes than bacteria grown with mono-unsaturated fatty acids. This was also the case for MLCL, but the numbers of double bonds for CL were quite similar for these three samples. The results indicate that L. acidophilus La-5 possesses a molecular mechanism for remodelling and optimizing the fatty acid composition of CL and MLCL species and the molar ratio of CL and MLCL. This study contributes new knowledge on the previously uninvestigated lipidome of L. acidophilus La-5.

AB - Probiotics are bacteria used in the food industry due to their potential health benefits. In this study, the plasma membrane of the probiotic Lactobacillus acidophilus La-5 was investigated using state-of-the-art high-resolution shotgun lipidomics. Comparisons of the lipidome of the plasma membrane were done after altering the fatty acid composition by supplementing L. acidophilus La-5 with saturated, mono-, di- and tri-unsaturated fatty acids during fermentation. The plasma membrane with the highest degree of saturation resulted in a lipid composition with the highest proportion of cardiolipin (CL) and lowest proportion of monolysocardiolipin (MLCL). No significant changes were found for other lipid classes. The bacteria grown with di- and tri-unsaturated fatty acids were expected to have more unsaturated plasma membranes than bacteria grown with mono-unsaturated fatty acids. This was also the case for MLCL, but the numbers of double bonds for CL were quite similar for these three samples. The results indicate that L. acidophilus La-5 possesses a molecular mechanism for remodelling and optimizing the fatty acid composition of CL and MLCL species and the molar ratio of CL and MLCL. This study contributes new knowledge on the previously uninvestigated lipidome of L. acidophilus La-5.

U2 - 10.1099/mic.0.000145

DO - 10.1099/mic.0.000145

M3 - Journal article

C2 - 26197785

VL - 161

SP - 1990

EP - 1998

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 10

ER -

ID: 147626220