Modification-specific proteomic analysis of glycoproteins in human body fluids by mass spectrometry

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Glycosylation of proteins is a very common, diverse, and heterogeneous type of modification, especially for proteins with extracellular destinations. This chapter describes some general strategies for the enrichment of glycoproteins and glycopeptides with an emphasis on proteomic analysis of N-glycosylated proteins in body fluids and other complex samples. An approach for identification of N-glycosylated proteins and mapping of their glycosylation sites is described. In this approach, glycoproteins are initially selectively purified by lectin chromatography. Following tryptic digestion, glycopeptides are enriched by hydrophilic interaction chromatography (HILIC). Glycan heterogeneity is then reduced by treating the glycopeptides with endoglycosidases. The resulting peptides are then analyzed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry and nano-flow reversed-phase liquid chromatography tandem mass spectrometry (LC-MS/MS). The analysis allows the identification of N-glycosylation sites and is demonstrated on a mixture of standard proteins.

Original languageEnglish
Title of host publicationProteomics of Human Body Fluids : Principles, Methods, and Applications
Number of pages22
PublisherHumana Press
Publication date1 Jan 2007
ISBN (Print)9781588296573
ISBN (Electronic)9781597454322
Publication statusPublished - 1 Jan 2007

    Research areas

  • glycoproteomics, glycosylation, HILIC, lectin, mass spectrometry, plasma proteins, posttranslational modifications, Proteomics

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