Modification of T-cell antigenic properties of tetanus toxoid by SDS-PAGE separation. Implications for T-cell blotting

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Standard

Modification of T-cell antigenic properties of tetanus toxoid by SDS-PAGE separation. Implications for T-cell blotting. / Christensen, C B; Theander, T G.

In: Journal of Immunoassay and Immunochemistry, Vol. 18, No. 2, 1997, p. 129-48.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Christensen, CB & Theander, TG 1997, 'Modification of T-cell antigenic properties of tetanus toxoid by SDS-PAGE separation. Implications for T-cell blotting', Journal of Immunoassay and Immunochemistry, vol. 18, no. 2, pp. 129-48.

APA

Christensen, C. B., & Theander, T. G. (1997). Modification of T-cell antigenic properties of tetanus toxoid by SDS-PAGE separation. Implications for T-cell blotting. Journal of Immunoassay and Immunochemistry, 18(2), 129-48.

Vancouver

Christensen CB, Theander TG. Modification of T-cell antigenic properties of tetanus toxoid by SDS-PAGE separation. Implications for T-cell blotting. Journal of Immunoassay and Immunochemistry. 1997;18(2):129-48.

Author

Christensen, C B ; Theander, T G. / Modification of T-cell antigenic properties of tetanus toxoid by SDS-PAGE separation. Implications for T-cell blotting. In: Journal of Immunoassay and Immunochemistry. 1997 ; Vol. 18, No. 2. pp. 129-48.

Bibtex

@article{59e0e040a0d911dd86a6000ea68e967b,
title = "Modification of T-cell antigenic properties of tetanus toxoid by SDS-PAGE separation. Implications for T-cell blotting",
abstract = "Using Tetanus Toxoid (TT) as a model antigen the T-cell Blotting method was evaluated. Peripheral blood mononuclear cell (PBMC) cultures were stimulated by blotted nitrocellulose-bound TT or soluble TT. SDS-Poly-Acrylamide-Gel-Electrophoresis separated TT only induced proliferation in 20% of the PBMC cultures whereas proliferation was induced in 79% of the same cultures offered similar treated TT (except for the PAGE separation). When T-cell blotting was performed with TT separated in a SDS-agarose matrix, proliferation was induced in 80% of donors responding to soluble TT. The results show that SDS-PAGE alters the ability of TT to induce T-cell proliferation, possibly due to unpolymerized acrylamide binding to proteins during SDS-PAGE. The use of SDS-PAGE T-cell blotting in the screening for T-cell antigens must therefore be reconsidered. We suggest the use of SDS-Agarose Gel Electrophoresis as an alternative when doing T-cell blots.",
author = "Christensen, {C B} and Theander, {T G}",
note = "Keywords: Acrylamide; Acrylamides; Collodion; Electrophoresis, Agar Gel; Electrophoresis, Polyacrylamide Gel; Epitopes, T-Lymphocyte; Humans; Immunoblotting; Lymphocyte Activation; Solubility; T-Lymphocytes; Tetanus Toxoid",
year = "1997",
language = "English",
volume = "18",
pages = "129--48",
journal = "Journal of Immunoassay and Immunochemistry",
issn = "1532-1819",
publisher = "Taylor & Francis",
number = "2",

}

RIS

TY - JOUR

T1 - Modification of T-cell antigenic properties of tetanus toxoid by SDS-PAGE separation. Implications for T-cell blotting

AU - Christensen, C B

AU - Theander, T G

N1 - Keywords: Acrylamide; Acrylamides; Collodion; Electrophoresis, Agar Gel; Electrophoresis, Polyacrylamide Gel; Epitopes, T-Lymphocyte; Humans; Immunoblotting; Lymphocyte Activation; Solubility; T-Lymphocytes; Tetanus Toxoid

PY - 1997

Y1 - 1997

N2 - Using Tetanus Toxoid (TT) as a model antigen the T-cell Blotting method was evaluated. Peripheral blood mononuclear cell (PBMC) cultures were stimulated by blotted nitrocellulose-bound TT or soluble TT. SDS-Poly-Acrylamide-Gel-Electrophoresis separated TT only induced proliferation in 20% of the PBMC cultures whereas proliferation was induced in 79% of the same cultures offered similar treated TT (except for the PAGE separation). When T-cell blotting was performed with TT separated in a SDS-agarose matrix, proliferation was induced in 80% of donors responding to soluble TT. The results show that SDS-PAGE alters the ability of TT to induce T-cell proliferation, possibly due to unpolymerized acrylamide binding to proteins during SDS-PAGE. The use of SDS-PAGE T-cell blotting in the screening for T-cell antigens must therefore be reconsidered. We suggest the use of SDS-Agarose Gel Electrophoresis as an alternative when doing T-cell blots.

AB - Using Tetanus Toxoid (TT) as a model antigen the T-cell Blotting method was evaluated. Peripheral blood mononuclear cell (PBMC) cultures were stimulated by blotted nitrocellulose-bound TT or soluble TT. SDS-Poly-Acrylamide-Gel-Electrophoresis separated TT only induced proliferation in 20% of the PBMC cultures whereas proliferation was induced in 79% of the same cultures offered similar treated TT (except for the PAGE separation). When T-cell blotting was performed with TT separated in a SDS-agarose matrix, proliferation was induced in 80% of donors responding to soluble TT. The results show that SDS-PAGE alters the ability of TT to induce T-cell proliferation, possibly due to unpolymerized acrylamide binding to proteins during SDS-PAGE. The use of SDS-PAGE T-cell blotting in the screening for T-cell antigens must therefore be reconsidered. We suggest the use of SDS-Agarose Gel Electrophoresis as an alternative when doing T-cell blots.

M3 - Journal article

C2 - 9134473

VL - 18

SP - 129

EP - 148

JO - Journal of Immunoassay and Immunochemistry

JF - Journal of Immunoassay and Immunochemistry

SN - 1532-1819

IS - 2

ER -

ID: 6766257