Mitochondrial oxidative phosphorylation capacity of cryopreserved cells
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Mitochondrial oxidative phosphorylation capacity of cryopreserved cells. / Lauridsen, Poul Ejner; Rasmussen, Lene Juel; Desler, Claus.
In: Mitochondrion, Vol. 47, 2019, p. 47-53.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Mitochondrial oxidative phosphorylation capacity of cryopreserved cells
AU - Lauridsen, Poul Ejner
AU - Rasmussen, Lene Juel
AU - Desler, Claus
PY - 2019
Y1 - 2019
N2 - Defects in mitochondrial oxidative phosphorylation are a feature of many human diseases. To date, determination of oxidative phosphorylation has required fresh and live sample material and therefore also access to specialized equipment and trained personnel. Cryopreservation of samples is an attractive alternative, where samples can be collected and stored in an economic and practical fashion for later bulk assays. Here, we present an accurate, reliable method for estimating mitochondrial oxidative phosphorylation capacity of cryopreserved human cells. Broad adoption of this method will allow uncomplicated collection of samples and measurements of oxidative phosphorylation.
AB - Defects in mitochondrial oxidative phosphorylation are a feature of many human diseases. To date, determination of oxidative phosphorylation has required fresh and live sample material and therefore also access to specialized equipment and trained personnel. Cryopreservation of samples is an attractive alternative, where samples can be collected and stored in an economic and practical fashion for later bulk assays. Here, we present an accurate, reliable method for estimating mitochondrial oxidative phosphorylation capacity of cryopreserved human cells. Broad adoption of this method will allow uncomplicated collection of samples and measurements of oxidative phosphorylation.
KW - Bioenergetics
KW - Cryopreservation
KW - Immuno-detection
KW - Oxidative phosphorylation
U2 - 10.1016/j.mito.2019.04.011
DO - 10.1016/j.mito.2019.04.011
M3 - Journal article
C2 - 31051261
AN - SCOPUS:85065493789
VL - 47
SP - 47
EP - 53
JO - Mitochondrion
JF - Mitochondrion
SN - 1567-7249
ER -
ID: 226792163