Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

Research output: Contribution to journal › Review › Research › peer-review

Standard

Minimum Information for Reporting on the Comet Assay (MIRCA) : recommendations for describing comet assay procedures and results. / Møller, Peter; Azqueta, Amaya; Boutet-Robinet, Elisa; Koppen, Gudrun; Bonassi, Stefano; Milić, Mirta; Gajski, Goran; Costa, Solange; Teixeira, João Paulo; Costa Pereira, Cristiana; Dusinska, Maria; Godschalk, Roger; Brunborg, Gunnar; Gutzkow, Kristine B.; Giovannelli, Lisa; Cooke, Marcus S.; Richling, Elke; Laffon, Blanca; Valdiglesias, Vanessa; Basaran, Nursen; Del Bo’, Cristian; Zegura, Bojana; Novak, Matjaz; Stopper, Helga; Vodicka, Pavel; Vodenkova, Sona; de Andrade, Vanessa Moraes; Sramkova, Monika; Gabelova, Alena; Collins, Andrew; Langie, Sabine A.S.

In: Nature Protocols, Vol. 15, 2020, p. 3817-3826.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Møller, P, Azqueta, A, Boutet-Robinet, E, Koppen, G, Bonassi, S, Milić, M, Gajski, G, Costa, S, Teixeira, JP, Costa Pereira, C, Dusinska, M, Godschalk, R, Brunborg, G, Gutzkow, KB, Giovannelli, L, Cooke, MS, Richling, E, Laffon, B, Valdiglesias, V, Basaran, N, Del Bo’, C, Zegura, B, Novak, M, Stopper, H, Vodicka, P, Vodenkova, S, de Andrade, VM, Sramkova, M, Gabelova, A, Collins, A & Langie, SAS 2020, 'Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results', Nature Protocols, vol. 15, pp. 3817-3826. https://doi.org/10.1038/s41596-020-0398-1

APA

Møller, P., Azqueta, A., Boutet-Robinet, E., Koppen, G., Bonassi, S., Milić, M., Gajski, G., Costa, S., Teixeira, J. P., Costa Pereira, C., Dusinska, M., Godschalk, R., Brunborg, G., Gutzkow, K. B., Giovannelli, L., Cooke, M. S., Richling, E., Laffon, B., Valdiglesias, V., ... Langie, S. A. S. (2020). Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. Nature Protocols, 15, 3817-3826. https://doi.org/10.1038/s41596-020-0398-1

Vancouver

Møller P, Azqueta A, Boutet-Robinet E, Koppen G, Bonassi S, Milić M et al. Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. Nature Protocols. 2020;15:3817-3826. https://doi.org/10.1038/s41596-020-0398-1

Author

Møller, Peter ; Azqueta, Amaya ; Boutet-Robinet, Elisa ; Koppen, Gudrun ; Bonassi, Stefano ; Milić, Mirta ; Gajski, Goran ; Costa, Solange ; Teixeira, João Paulo ; Costa Pereira, Cristiana ; Dusinska, Maria ; Godschalk, Roger ; Brunborg, Gunnar ; Gutzkow, Kristine B. ; Giovannelli, Lisa ; Cooke, Marcus S. ; Richling, Elke ; Laffon, Blanca ; Valdiglesias, Vanessa ; Basaran, Nursen ; Del Bo’, Cristian ; Zegura, Bojana ; Novak, Matjaz ; Stopper, Helga ; Vodicka, Pavel ; Vodenkova, Sona ; de Andrade, Vanessa Moraes ; Sramkova, Monika ; Gabelova, Alena ; Collins, Andrew ; Langie, Sabine A.S. / Minimum Information for Reporting on the Comet Assay (MIRCA) : recommendations for describing comet assay procedures and results. In: Nature Protocols. 2020 ; Vol. 15. pp. 3817-3826.

Bibtex

@article{196b00a098e94b4ca7a1297cddb3df11,
title = "Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results",
abstract = "The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between {\textquoteleft}desirable{\textquoteright} and {\textquoteleft}essential{\textquoteright} information: {\textquoteleft}essential{\textquoteright} information refers to the precise details that are necessary to assess the quality of the experimental work, whereas {\textquoteleft}desirable{\textquoteright} information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.",
author = "Peter M{\o}ller and Amaya Azqueta and Elisa Boutet-Robinet and Gudrun Koppen and Stefano Bonassi and Mirta Mili{\'c} and Goran Gajski and Solange Costa and Teixeira, {Jo{\~a}o Paulo} and {Costa Pereira}, Cristiana and Maria Dusinska and Roger Godschalk and Gunnar Brunborg and Gutzkow, {Kristine B.} and Lisa Giovannelli and Cooke, {Marcus S.} and Elke Richling and Blanca Laffon and Vanessa Valdiglesias and Nursen Basaran and {Del Bo{\textquoteright}}, Cristian and Bojana Zegura and Matjaz Novak and Helga Stopper and Pavel Vodicka and Sona Vodenkova and {de Andrade}, {Vanessa Moraes} and Monika Sramkova and Alena Gabelova and Andrew Collins and Langie, {Sabine A.S.}",
year = "2020",
doi = "10.1038/s41596-020-0398-1",
language = "English",
volume = "15",
pages = "3817--3826",
journal = "Nature Protocols",
issn = "1754-2189",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Minimum Information for Reporting on the Comet Assay (MIRCA)

T2 - recommendations for describing comet assay procedures and results

AU - Møller, Peter

AU - Azqueta, Amaya

AU - Boutet-Robinet, Elisa

AU - Koppen, Gudrun

AU - Bonassi, Stefano

AU - Milić, Mirta

AU - Gajski, Goran

AU - Costa, Solange

AU - Teixeira, João Paulo

AU - Costa Pereira, Cristiana

AU - Dusinska, Maria

AU - Godschalk, Roger

AU - Brunborg, Gunnar

AU - Gutzkow, Kristine B.

AU - Giovannelli, Lisa

AU - Cooke, Marcus S.

AU - Richling, Elke

AU - Laffon, Blanca

AU - Valdiglesias, Vanessa

AU - Basaran, Nursen

AU - Del Bo’, Cristian

AU - Zegura, Bojana

AU - Novak, Matjaz

AU - Stopper, Helga

AU - Vodicka, Pavel

AU - Vodenkova, Sona

AU - de Andrade, Vanessa Moraes

AU - Sramkova, Monika

AU - Gabelova, Alena

AU - Collins, Andrew

AU - Langie, Sabine A.S.

PY - 2020

Y1 - 2020

N2 - The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between ‘desirable’ and ‘essential’ information: ‘essential’ information refers to the precise details that are necessary to assess the quality of the experimental work, whereas ‘desirable’ information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.

AB - The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between ‘desirable’ and ‘essential’ information: ‘essential’ information refers to the precise details that are necessary to assess the quality of the experimental work, whereas ‘desirable’ information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.

U2 - 10.1038/s41596-020-0398-1

DO - 10.1038/s41596-020-0398-1

M3 - Review

C2 - 33106678

AN - SCOPUS:85093963487

VL - 15

SP - 3817

EP - 3826

JO - Nature Protocols

JF - Nature Protocols

SN - 1754-2189

ER -

ID: 251409094