Microfluidic-Derived Detection of Protein-Facilitated Copper Flux Across Lipid Membranes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Microfluidic-Derived Detection of Protein-Facilitated Copper Flux Across Lipid Membranes. / Gorecki, Kamil; Hansen, Jesper S.; Li, Ping; Nayeri, Niloofar; Lindkvist-Petersson, Karin; Gourdon, Pontus.

In: Analytical Chemistry, Vol. 94, No. 34, 2022, p. 11831-11837.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Gorecki, K, Hansen, JS, Li, P, Nayeri, N, Lindkvist-Petersson, K & Gourdon, P 2022, 'Microfluidic-Derived Detection of Protein-Facilitated Copper Flux Across Lipid Membranes', Analytical Chemistry, vol. 94, no. 34, pp. 11831-11837. https://doi.org/10.1021/acs.analchem.2c02081

APA

Gorecki, K., Hansen, J. S., Li, P., Nayeri, N., Lindkvist-Petersson, K., & Gourdon, P. (2022). Microfluidic-Derived Detection of Protein-Facilitated Copper Flux Across Lipid Membranes. Analytical Chemistry, 94(34), 11831-11837. https://doi.org/10.1021/acs.analchem.2c02081

Vancouver

Gorecki K, Hansen JS, Li P, Nayeri N, Lindkvist-Petersson K, Gourdon P. Microfluidic-Derived Detection of Protein-Facilitated Copper Flux Across Lipid Membranes. Analytical Chemistry. 2022;94(34):11831-11837. https://doi.org/10.1021/acs.analchem.2c02081

Author

Gorecki, Kamil ; Hansen, Jesper S. ; Li, Ping ; Nayeri, Niloofar ; Lindkvist-Petersson, Karin ; Gourdon, Pontus. / Microfluidic-Derived Detection of Protein-Facilitated Copper Flux Across Lipid Membranes. In: Analytical Chemistry. 2022 ; Vol. 94, No. 34. pp. 11831-11837.

Bibtex

@article{ebce3d5db55b42dabf037e42891875d9,

title = "Microfluidic-Derived Detection of Protein-Facilitated Copper Flux Across Lipid Membranes",

abstract = "Measurement of protein-facilitated copper flux across biological membranes is a considerable challenge. Here, we demonstrate a straightforward microfluidic-derived approach for visualization and measurement of membranous Cu flux. Giant unilamellar vesicles, reconstituted with the membrane protein of interest, are prepared, surface-immobilized, and assessed using a novel quencher-sensor reporter system for detection of copper. With the aid of a syringe pump, the external buffer is exchanged, enabling consistent and precise exchange of solutes, without causing vesicle rupture or uneven local metal concentrations brought about by rapid mixing. This approach bypasses common issues encountered when studying heavy metal-ion flux, thereby providing a new platform for in vitro studies of metal homeostasis aspects that are critical for all cells, health, and disease.",

keywords = "RESISTANCE, TRANSPORT, RECONSTITUTION, HOMEOSTASIS, COPA",

author = "Kamil Gorecki and Hansen, {Jesper S.} and Ping Li and Niloofar Nayeri and Karin Lindkvist-Petersson and Pontus Gourdon",

year = "2022",

doi = "10.1021/acs.analchem.2c02081",

language = "English",

volume = "94",

pages = "11831--11837",

journal = "Industrial And Engineering Chemistry Analytical Edition",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "34",

}

RIS

TY - JOUR

T1 - Microfluidic-Derived Detection of Protein-Facilitated Copper Flux Across Lipid Membranes

AU - Gorecki, Kamil

AU - Hansen, Jesper S.

AU - Li, Ping

AU - Nayeri, Niloofar

AU - Lindkvist-Petersson, Karin

AU - Gourdon, Pontus

PY - 2022

Y1 - 2022

N2 - Measurement of protein-facilitated copper flux across biological membranes is a considerable challenge. Here, we demonstrate a straightforward microfluidic-derived approach for visualization and measurement of membranous Cu flux. Giant unilamellar vesicles, reconstituted with the membrane protein of interest, are prepared, surface-immobilized, and assessed using a novel quencher-sensor reporter system for detection of copper. With the aid of a syringe pump, the external buffer is exchanged, enabling consistent and precise exchange of solutes, without causing vesicle rupture or uneven local metal concentrations brought about by rapid mixing. This approach bypasses common issues encountered when studying heavy metal-ion flux, thereby providing a new platform for in vitro studies of metal homeostasis aspects that are critical for all cells, health, and disease.

AB - Measurement of protein-facilitated copper flux across biological membranes is a considerable challenge. Here, we demonstrate a straightforward microfluidic-derived approach for visualization and measurement of membranous Cu flux. Giant unilamellar vesicles, reconstituted with the membrane protein of interest, are prepared, surface-immobilized, and assessed using a novel quencher-sensor reporter system for detection of copper. With the aid of a syringe pump, the external buffer is exchanged, enabling consistent and precise exchange of solutes, without causing vesicle rupture or uneven local metal concentrations brought about by rapid mixing. This approach bypasses common issues encountered when studying heavy metal-ion flux, thereby providing a new platform for in vitro studies of metal homeostasis aspects that are critical for all cells, health, and disease.

KW - RESISTANCE

KW - TRANSPORT

KW - RECONSTITUTION

KW - HOMEOSTASIS

KW - COPA

U2 - 10.1021/acs.analchem.2c02081

DO - 10.1021/acs.analchem.2c02081

M3 - Journal article

C2 - 35969432

VL - 94

SP - 11831

EP - 11837

JO - Industrial And Engineering Chemistry Analytical Edition

JF - Industrial And Engineering Chemistry Analytical Edition

SN - 0003-2700

IS - 34

ER -

ID: 320393650