Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production
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Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. / Alonso-Gutierrez, Jorge; Chan, Rossana; Batth, Tanveer S; Adams, Paul D; Keasling, Jay D; Petzold, Christopher J; Lee, Taek Soon.
In: Metabolic Engineering, Vol. 19, 09.2013, p. 33-41.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production
AU - Alonso-Gutierrez, Jorge
AU - Chan, Rossana
AU - Batth, Tanveer S
AU - Adams, Paul D
AU - Keasling, Jay D
AU - Petzold, Christopher J
AU - Lee, Taek Soon
N1 - © 2013 Elsevier Inc. All rights reserved.
PY - 2013/9
Y1 - 2013/9
N2 - Limonene is a valuable monoterpene used in the production of several commodity chemicals and medicinal compounds. Among them, perillyl alcohol (POH) is a promising anti-cancer agent that can be produced by hydroxylation of limonene. We engineered E. coli with a heterologous mevalonate pathway and limonene synthase for production of limonene followed by coupling with a cytochrome P450, which specifically hydroxylates limonene to produce POH. A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past. Incorporation of a cytochrome P450 to hydroxylate limonene yielded approximately 100mg/L of POH. Further metabolic engineering of the pathway and in situ product recovery using anion exchange resins would make this engineered E. coli a potential production platform for any valuable limonene derivative.
AB - Limonene is a valuable monoterpene used in the production of several commodity chemicals and medicinal compounds. Among them, perillyl alcohol (POH) is a promising anti-cancer agent that can be produced by hydroxylation of limonene. We engineered E. coli with a heterologous mevalonate pathway and limonene synthase for production of limonene followed by coupling with a cytochrome P450, which specifically hydroxylates limonene to produce POH. A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past. Incorporation of a cytochrome P450 to hydroxylate limonene yielded approximately 100mg/L of POH. Further metabolic engineering of the pathway and in situ product recovery using anion exchange resins would make this engineered E. coli a potential production platform for any valuable limonene derivative.
U2 - 10.1016/j.ymben.2013.05.004
DO - 10.1016/j.ymben.2013.05.004
M3 - Journal article
C2 - 23727191
VL - 19
SP - 33
EP - 41
JO - Metabolic Engineering
JF - Metabolic Engineering
SN - 1096-7176
ER -
ID: 68162550