MANF Promotes Differentiation and Migration of Neural Progenitor Cells with Potential Neural Regenerative Effects in Stroke

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

MANF Promotes Differentiation and Migration of Neural Progenitor Cells with Potential Neural Regenerative Effects in Stroke. / Tseng, Kuan-Yin; Anttila, Jenni E; Khodosevich, Konstantin; Tuominen, Raimo K; Lindahl, Maria; Domanskyi, Andrii; Airavaara, Mikko.

In: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 26, No. 1, 2018, p. 238-255.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Tseng, K-Y, Anttila, JE, Khodosevich, K, Tuominen, RK, Lindahl, M, Domanskyi, A & Airavaara, M 2018, 'MANF Promotes Differentiation and Migration of Neural Progenitor Cells with Potential Neural Regenerative Effects in Stroke', Molecular therapy : the journal of the American Society of Gene Therapy, vol. 26, no. 1, pp. 238-255. https://doi.org/10.1016/j.ymthe.2017.09.019

APA

Tseng, K-Y., Anttila, J. E., Khodosevich, K., Tuominen, R. K., Lindahl, M., Domanskyi, A., & Airavaara, M. (2018). MANF Promotes Differentiation and Migration of Neural Progenitor Cells with Potential Neural Regenerative Effects in Stroke. Molecular therapy : the journal of the American Society of Gene Therapy, 26(1), 238-255. https://doi.org/10.1016/j.ymthe.2017.09.019

Vancouver

Tseng K-Y, Anttila JE, Khodosevich K, Tuominen RK, Lindahl M, Domanskyi A et al. MANF Promotes Differentiation and Migration of Neural Progenitor Cells with Potential Neural Regenerative Effects in Stroke. Molecular therapy : the journal of the American Society of Gene Therapy. 2018;26(1):238-255. https://doi.org/10.1016/j.ymthe.2017.09.019

Author

Tseng, Kuan-Yin ; Anttila, Jenni E ; Khodosevich, Konstantin ; Tuominen, Raimo K ; Lindahl, Maria ; Domanskyi, Andrii ; Airavaara, Mikko. / MANF Promotes Differentiation and Migration of Neural Progenitor Cells with Potential Neural Regenerative Effects in Stroke. In: Molecular therapy : the journal of the American Society of Gene Therapy. 2018 ; Vol. 26, No. 1. pp. 238-255.

Bibtex

@article{534e93458ea34de9bb56f4efc73c6626,

title = "MANF Promotes Differentiation and Migration of Neural Progenitor Cells with Potential Neural Regenerative Effects in Stroke",

abstract = "Cerebral ischemia activates endogenous reparative processes, such as increased proliferation of neural stem cells (NSCs) in the subventricular zone (SVZ) and migration of neural progenitor cells (NPCs) toward the ischemic area. However, this reparative process is limited because most of the NPCs die shortly after injury or are unable to arrive at the infarct boundary. In this study, we demonstrate for the first time that endogenous mesencephalic astrocyte-derived neurotrophic factor (MANF) protects NSCs against oxygen-glucose-deprivation-induced injury and has a crucial role in regulating NPC migration. In NSC cultures, MANF protein administration did not affect growth of cells but triggered neuronal and glial differentiation, followed by activation of STAT3. In SVZ explants, MANF overexpression facilitated cell migration and activated the STAT3 and ERK1/2 pathway. Using a rat model of cortical stroke, intracerebroventricular injections of MANF did not affect cell proliferation in the SVZ, but promoted migration of doublecortin (DCX)+ cells toward the corpus callosum and infarct boundary on day 14 post-stroke. Long-term infusion of MANF into the peri-infarct zone increased the recruitment of DCX+ cells in the infarct area. In conclusion, our data demonstrate a neuroregenerative activity of MANF that facilitates differentiation and migration of NPCs, thereby increasing recruitment of neuroblasts in stroke cortex.",

keywords = "Journal Article",

author = "Kuan-Yin Tseng and Anttila, {Jenni E} and Konstantin Khodosevich and Tuominen, {Raimo K} and Maria Lindahl and Andrii Domanskyi and Mikko Airavaara",

year = "2018",

doi = "10.1016/j.ymthe.2017.09.019",

language = "English",

volume = "26",

pages = "238--255",

journal = "Molecular Therapy",

issn = "1525-0016",

publisher = "nature publishing group",

number = "1",

}

RIS

TY - JOUR

T1 - MANF Promotes Differentiation and Migration of Neural Progenitor Cells with Potential Neural Regenerative Effects in Stroke

AU - Tseng, Kuan-Yin

AU - Anttila, Jenni E

AU - Khodosevich, Konstantin

AU - Tuominen, Raimo K

AU - Lindahl, Maria

AU - Domanskyi, Andrii

AU - Airavaara, Mikko

PY - 2018

Y1 - 2018

N2 - Cerebral ischemia activates endogenous reparative processes, such as increased proliferation of neural stem cells (NSCs) in the subventricular zone (SVZ) and migration of neural progenitor cells (NPCs) toward the ischemic area. However, this reparative process is limited because most of the NPCs die shortly after injury or are unable to arrive at the infarct boundary. In this study, we demonstrate for the first time that endogenous mesencephalic astrocyte-derived neurotrophic factor (MANF) protects NSCs against oxygen-glucose-deprivation-induced injury and has a crucial role in regulating NPC migration. In NSC cultures, MANF protein administration did not affect growth of cells but triggered neuronal and glial differentiation, followed by activation of STAT3. In SVZ explants, MANF overexpression facilitated cell migration and activated the STAT3 and ERK1/2 pathway. Using a rat model of cortical stroke, intracerebroventricular injections of MANF did not affect cell proliferation in the SVZ, but promoted migration of doublecortin (DCX)+ cells toward the corpus callosum and infarct boundary on day 14 post-stroke. Long-term infusion of MANF into the peri-infarct zone increased the recruitment of DCX+ cells in the infarct area. In conclusion, our data demonstrate a neuroregenerative activity of MANF that facilitates differentiation and migration of NPCs, thereby increasing recruitment of neuroblasts in stroke cortex.

AB - Cerebral ischemia activates endogenous reparative processes, such as increased proliferation of neural stem cells (NSCs) in the subventricular zone (SVZ) and migration of neural progenitor cells (NPCs) toward the ischemic area. However, this reparative process is limited because most of the NPCs die shortly after injury or are unable to arrive at the infarct boundary. In this study, we demonstrate for the first time that endogenous mesencephalic astrocyte-derived neurotrophic factor (MANF) protects NSCs against oxygen-glucose-deprivation-induced injury and has a crucial role in regulating NPC migration. In NSC cultures, MANF protein administration did not affect growth of cells but triggered neuronal and glial differentiation, followed by activation of STAT3. In SVZ explants, MANF overexpression facilitated cell migration and activated the STAT3 and ERK1/2 pathway. Using a rat model of cortical stroke, intracerebroventricular injections of MANF did not affect cell proliferation in the SVZ, but promoted migration of doublecortin (DCX)+ cells toward the corpus callosum and infarct boundary on day 14 post-stroke. Long-term infusion of MANF into the peri-infarct zone increased the recruitment of DCX+ cells in the infarct area. In conclusion, our data demonstrate a neuroregenerative activity of MANF that facilitates differentiation and migration of NPCs, thereby increasing recruitment of neuroblasts in stroke cortex.

KW - Journal Article

U2 - 10.1016/j.ymthe.2017.09.019

DO - 10.1016/j.ymthe.2017.09.019

M3 - Journal article

C2 - 29050872

VL - 26

SP - 238

EP - 255

JO - Molecular Therapy

JF - Molecular Therapy

SN - 1525-0016

IS - 1

ER -

ID: 186874692