LPS-induced cytokine production in the monocytic cell line THP-1 determined by multiple quantitative competitive PCR (QC-PCR)
Research output: Contribution to journal › Journal article › Research › peer-review
Quantifying cytokines on the protein level can be problematic because of low concentrations or degradation during sample handling. Aiming towards finding a simple method by which to quantify cytokines on the mRNA level, we combined existing and established molecular biology techniques. Based on the principle of quantitative competitive RT-PCR with a DNA-competitor, IL-1beta, IL-6, IL-12alpha and the housekeeping enzyme GAPDH are measured at levels down to 200 copies of mRNA.
| Original language | English |
|---|---|
| Journal | Scandinavian Journal of Clinical & Laboratory Investigation |
| Volume | 62 |
| Issue number | 6 |
| Pages (from-to) | 405-12 |
| Number of pages | 8 |
| ISSN | 0036-5513 |
| Publication status | Published - 2002 |
- Cell Line, Cytokines, DNA, Complementary, Interleukin-1, Interleukin-12, Interleukin-6, Lipopolysaccharides, Monocytes, Polymerase Chain Reaction, RNA, Messenger
Research areas
ID: 50845198