Local application of FFR-rFVIIa reduces thrombus formation at arterial anastomosis in rats

Research output: Contribution to journalJournal articleResearchpeer-review

Thrombosis is still a significant problem in microvascular surgery. The aim of this study was to evaluate the antithrombotic effect of topically applied active site-inhibited recombinant human factor VIIa (FFR-rFVIIa) in a rat model with microvascular thrombosis. Forty-five male rats were allocated to one of three groups: local treatment with vehicle only, local treatment with 0.035 mg of FFR-rFVIIa, or local treatment with 0.35 mg of FFR-rFVIIa. An arteriotomy was made in the right femoral artery. Ten minutes following topical application, a thrombogenic anastomosis was performed. Using a transilluminator, thrombus formation and anastomotic bleeding episodes were observed and registered for 40 min. Local application of FFR-rFVIIa resulted in a 85-90% reduction of thrombus formation in both treated groups compared to the control group, but the reduction was only statistically significant in the group treated with 0.035 mg of FFR-rFVIIa. An increased occurrence and duration of anastomotic bleeding episodes were observed in both FFR-rFVIIa-treated groups.

Original languageEnglish
Issue number8
Pages (from-to)369-73
Number of pages5
Publication statusPublished - 1999

    Research areas

  • Administration, Topical, Animals, Arteriovenous Anastomosis, Factor VII/administration & dosage, Factor VIIa, Fibrinolytic Agents/administration & dosage, Humans, Male, Microsurgery, Postoperative Hemorrhage/etiology, Rats, Rats, Wistar, Recombinant Proteins/administration & dosage, Thrombosis/prevention & control

ID: 243520873