Linkers, resins, and general procedures for solid-phase peptide synthesis

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This chapter describes the basic protocols for solid-phase peptide synthesis using the Fmoc group as the N-alpha-protecting group (Fmoc-SPPS). The chapter introduces resins and their handling, choice of linkers, and the most common methods for peptide chain assembly. The proper choice of resins and linkers for solid-phase synthesis is a key parameter for successful peptide synthesis. This chapter provides an overview of the most common and useful resins and linkers for the synthesis of peptides with C-terminal amides, carboxylic acids, and more. The chapter finishes with robust protocols for general solid-phase peptide synthesis, i.e., the standard operations.
Original languageEnglish
Title of host publicationPeptide synthesis and applications
EditorsKnud J. Jensen, Pernille T. Shelton, Søren L. Pedersen
Number of pages19
PublisherHumana Press
Publication date2013
ISBN (Print)978-1-62703-543-9
ISBN (Electronic)978-1-62703-544-6
Publication statusPublished - 2013
SeriesMethods in Molecular Biology

ID: 118886496