LFA-1 subunit expression in ulcerative colitis patients

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

LFA-1 subunit expression in ulcerative colitis patients. / Kirman, I.; Nielsen, O. H.

In: Digestive Diseases and Sciences, Vol. 41, No. 4, 14.05.1996, p. 670-676.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Kirman, I & Nielsen, OH 1996, 'LFA-1 subunit expression in ulcerative colitis patients', Digestive Diseases and Sciences, vol. 41, no. 4, pp. 670-676. https://doi.org/10.1007/BF02213121

APA

Kirman, I., & Nielsen, O. H. (1996). LFA-1 subunit expression in ulcerative colitis patients. Digestive Diseases and Sciences, 41(4), 670-676. https://doi.org/10.1007/BF02213121

Vancouver

Kirman I, Nielsen OH. LFA-1 subunit expression in ulcerative colitis patients. Digestive Diseases and Sciences. 1996 May 14;41(4):670-676. https://doi.org/10.1007/BF02213121

Author

Kirman, I. ; Nielsen, O. H. / LFA-1 subunit expression in ulcerative colitis patients. In: Digestive Diseases and Sciences. 1996 ; Vol. 41, No. 4. pp. 670-676.

Bibtex

@article{ec14942cb08a4e84b90b2e478c557f1d,
title = "LFA-1 subunit expression in ulcerative colitis patients",
abstract = "The adhesion molecule, lymphocyte function associated antigen 1 (LFA-1) consisting of two subunits, CD11a and CD18, mediates lymphocyte migration into tissue and cell effector functions. Previous observations showed no differences in LFA-1 expression by circulating lymphocytes between inflammatory bowel disease patients and controls. The aim of the present work was to study subsets of circulating LFA-1+ lymphocytes in ulcerative colitis (UC) patients versus healthy controls. Peripheral blood mononuclear cells were obtained from 16 UC patients and 10 healthy volunteers. The percentages of CD11a(lo), CD11a(hi); CD18(lo), CD18(hi) T and B cells, as well as CD25 expression on these cells were studied using double staining with monoclonal antibodies and panning procedures. The percentage of CD11(hi) and CD18(hi) T cells was significantly decreased in quiescent UC patients as compared to active disease patients and healthy controls (P < 0.05). The majority of CD25+ T cells were expressing CD11a and CD18 with low density. A detectable percentage, 2{\%} (range 1-6{\%}), of CD11a(hi)CD25+ (but not CD18(hi)CD25+) was found in UC patients with moderate to severe disease, but not in those with inactive UC or healthy controls. In conclusion, the percentage of CD11a(hi+) and CD18(hi+) T cells is decreased in peripheral blood of quiescent UC patients, which is probably associated with the effect of specific treatment. The percentage of CD11a(hi+)IL-2Rα+ T cells is increased in peripheral blood of patients with active (moderate and severe) UC, which most likely reflects a sustained T-cell activation due to a persistent inflammatory process.",
keywords = "adhesion molecules, lymphocyte function associated antigen-1, ulcerative colitis",
author = "I. Kirman and Nielsen, {O. H.}",
year = "1996",
month = "5",
day = "14",
doi = "10.1007/BF02213121",
language = "English",
volume = "41",
pages = "670--676",
journal = "Digestive Diseases and Sciences",
issn = "0163-2116",
publisher = "Springer",
number = "4",

}

RIS

TY - JOUR

T1 - LFA-1 subunit expression in ulcerative colitis patients

AU - Kirman, I.

AU - Nielsen, O. H.

PY - 1996/5/14

Y1 - 1996/5/14

N2 - The adhesion molecule, lymphocyte function associated antigen 1 (LFA-1) consisting of two subunits, CD11a and CD18, mediates lymphocyte migration into tissue and cell effector functions. Previous observations showed no differences in LFA-1 expression by circulating lymphocytes between inflammatory bowel disease patients and controls. The aim of the present work was to study subsets of circulating LFA-1+ lymphocytes in ulcerative colitis (UC) patients versus healthy controls. Peripheral blood mononuclear cells were obtained from 16 UC patients and 10 healthy volunteers. The percentages of CD11a(lo), CD11a(hi); CD18(lo), CD18(hi) T and B cells, as well as CD25 expression on these cells were studied using double staining with monoclonal antibodies and panning procedures. The percentage of CD11(hi) and CD18(hi) T cells was significantly decreased in quiescent UC patients as compared to active disease patients and healthy controls (P < 0.05). The majority of CD25+ T cells were expressing CD11a and CD18 with low density. A detectable percentage, 2% (range 1-6%), of CD11a(hi)CD25+ (but not CD18(hi)CD25+) was found in UC patients with moderate to severe disease, but not in those with inactive UC or healthy controls. In conclusion, the percentage of CD11a(hi+) and CD18(hi+) T cells is decreased in peripheral blood of quiescent UC patients, which is probably associated with the effect of specific treatment. The percentage of CD11a(hi+)IL-2Rα+ T cells is increased in peripheral blood of patients with active (moderate and severe) UC, which most likely reflects a sustained T-cell activation due to a persistent inflammatory process.

AB - The adhesion molecule, lymphocyte function associated antigen 1 (LFA-1) consisting of two subunits, CD11a and CD18, mediates lymphocyte migration into tissue and cell effector functions. Previous observations showed no differences in LFA-1 expression by circulating lymphocytes between inflammatory bowel disease patients and controls. The aim of the present work was to study subsets of circulating LFA-1+ lymphocytes in ulcerative colitis (UC) patients versus healthy controls. Peripheral blood mononuclear cells were obtained from 16 UC patients and 10 healthy volunteers. The percentages of CD11a(lo), CD11a(hi); CD18(lo), CD18(hi) T and B cells, as well as CD25 expression on these cells were studied using double staining with monoclonal antibodies and panning procedures. The percentage of CD11(hi) and CD18(hi) T cells was significantly decreased in quiescent UC patients as compared to active disease patients and healthy controls (P < 0.05). The majority of CD25+ T cells were expressing CD11a and CD18 with low density. A detectable percentage, 2% (range 1-6%), of CD11a(hi)CD25+ (but not CD18(hi)CD25+) was found in UC patients with moderate to severe disease, but not in those with inactive UC or healthy controls. In conclusion, the percentage of CD11a(hi+) and CD18(hi+) T cells is decreased in peripheral blood of quiescent UC patients, which is probably associated with the effect of specific treatment. The percentage of CD11a(hi+)IL-2Rα+ T cells is increased in peripheral blood of patients with active (moderate and severe) UC, which most likely reflects a sustained T-cell activation due to a persistent inflammatory process.

KW - adhesion molecules

KW - lymphocyte function associated antigen-1

KW - ulcerative colitis

UR - http://www.scopus.com/inward/record.url?scp=0029964289&partnerID=8YFLogxK

U2 - 10.1007/BF02213121

DO - 10.1007/BF02213121

M3 - Journal article

C2 - 8674386

AN - SCOPUS:0029964289

VL - 41

SP - 670

EP - 676

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 4

ER -

ID: 218718976