TY - JOUR

T1 - LFA-1 subunit expression in ulcerative colitis patients

AU - Kirman, I.

AU - Nielsen, O. H.

PY - 1996/5/14

Y1 - 1996/5/14

N2 - The adhesion molecule, lymphocyte function associated antigen 1 (LFA-1) consisting of two subunits, CD11a and CD18, mediates lymphocyte migration into tissue and cell effector functions. Previous observations showed no differences in LFA-1 expression by circulating lymphocytes between inflammatory bowel disease patients and controls. The aim of the present work was to study subsets of circulating LFA-1+ lymphocytes in ulcerative colitis (UC) patients versus healthy controls. Peripheral blood mononuclear cells were obtained from 16 UC patients and 10 healthy volunteers. The percentages of CD11a(lo), CD11a(hi); CD18(lo), CD18(hi) T and B cells, as well as CD25 expression on these cells were studied using double staining with monoclonal antibodies and panning procedures. The percentage of CD11(hi) and CD18(hi) T cells was significantly decreased in quiescent UC patients as compared to active disease patients and healthy controls (P < 0.05). The majority of CD25+ T cells were expressing CD11a and CD18 with low density. A detectable percentage, 2% (range 1-6%), of CD11a(hi)CD25+ (but not CD18(hi)CD25+) was found in UC patients with moderate to severe disease, but not in those with inactive UC or healthy controls. In conclusion, the percentage of CD11a(hi+) and CD18(hi+) T cells is decreased in peripheral blood of quiescent UC patients, which is probably associated with the effect of specific treatment. The percentage of CD11a(hi+)IL-2Rα+ T cells is increased in peripheral blood of patients with active (moderate and severe) UC, which most likely reflects a sustained T-cell activation due to a persistent inflammatory process.

AB - The adhesion molecule, lymphocyte function associated antigen 1 (LFA-1) consisting of two subunits, CD11a and CD18, mediates lymphocyte migration into tissue and cell effector functions. Previous observations showed no differences in LFA-1 expression by circulating lymphocytes between inflammatory bowel disease patients and controls. The aim of the present work was to study subsets of circulating LFA-1+ lymphocytes in ulcerative colitis (UC) patients versus healthy controls. Peripheral blood mononuclear cells were obtained from 16 UC patients and 10 healthy volunteers. The percentages of CD11a(lo), CD11a(hi); CD18(lo), CD18(hi) T and B cells, as well as CD25 expression on these cells were studied using double staining with monoclonal antibodies and panning procedures. The percentage of CD11(hi) and CD18(hi) T cells was significantly decreased in quiescent UC patients as compared to active disease patients and healthy controls (P < 0.05). The majority of CD25+ T cells were expressing CD11a and CD18 with low density. A detectable percentage, 2% (range 1-6%), of CD11a(hi)CD25+ (but not CD18(hi)CD25+) was found in UC patients with moderate to severe disease, but not in those with inactive UC or healthy controls. In conclusion, the percentage of CD11a(hi+) and CD18(hi+) T cells is decreased in peripheral blood of quiescent UC patients, which is probably associated with the effect of specific treatment. The percentage of CD11a(hi+)IL-2Rα+ T cells is increased in peripheral blood of patients with active (moderate and severe) UC, which most likely reflects a sustained T-cell activation due to a persistent inflammatory process.

KW - adhesion molecules

KW - lymphocyte function associated antigen-1

KW - ulcerative colitis

UR - http://www.scopus.com/inward/record.url?scp=0029964289&partnerID=8YFLogxK

U2 - 10.1007/BF02213121

DO - 10.1007/BF02213121

M3 - Journal article

C2 - 8674386

AN - SCOPUS:0029964289

VL - 41

SP - 670

EP - 676

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 4

ER -