Lactate dehydrogenase assay for assessment of polycation cytotoxicity

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research

Standard

Lactate dehydrogenase assay for assessment of polycation cytotoxicity. / Parhamifar, Ladan; Andersen, Helene; Moghimi, Seyed Moien.

Nanotechnology for Nucleic Acid Delivery. Vol. 948 2013. p. 13-22 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research

Harvard

Parhamifar, L, Andersen, H & Moghimi, SM 2013, Lactate dehydrogenase assay for assessment of polycation cytotoxicity. in Nanotechnology for Nucleic Acid Delivery. vol. 948, Methods in Molecular Biology, pp. 13-22. https://doi.org/10.1007/978-1-62703-140-0_2

APA

Parhamifar, L., Andersen, H., & Moghimi, S. M. (2013). Lactate dehydrogenase assay for assessment of polycation cytotoxicity. In Nanotechnology for Nucleic Acid Delivery (Vol. 948, pp. 13-22). Methods in Molecular Biology https://doi.org/10.1007/978-1-62703-140-0_2

Vancouver

Parhamifar L, Andersen H, Moghimi SM. Lactate dehydrogenase assay for assessment of polycation cytotoxicity. In Nanotechnology for Nucleic Acid Delivery. Vol. 948. 2013. p. 13-22. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-140-0_2

Author

Parhamifar, Ladan ; Andersen, Helene ; Moghimi, Seyed Moien. / Lactate dehydrogenase assay for assessment of polycation cytotoxicity. Nanotechnology for Nucleic Acid Delivery. Vol. 948 2013. pp. 13-22 (Methods in Molecular Biology).

Bibtex

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title = "Lactate dehydrogenase assay for assessment of polycation cytotoxicity",
abstract = "Cellular toxicity and/or cell death entail complex mechanisms that require detailed evaluation for proper characterization. A detailed mechanistic assessment of cytotoxicity is essential for design and construction of more effective polycations for nucleic acid delivery. A single toxicity assay cannot stand alone in determining the type and extent of damage or cell death mechanism. In this chapter we describe a lactate dehydrogenase (LDH) assay for high-throughput screening that can be used as a starting point for further detailed cytotoxicity determination. LDH release is considered an early event in necrosis but a late event in apoptosis. An accurate temporal assessment of the toxic responses is crucial as late apoptosis may convert into necrosis as well as in situations where cell death is initiated without any visible cell morphological changes or responses in assays measuring late events, resulting in early ongoing toxicity being overlooked.",
keywords = "Former Faculty of Pharmaceutical Sciences",
author = "Ladan Parhamifar and Helene Andersen and Moghimi, {Seyed Moien}",
year = "2013",
doi = "10.1007/978-1-62703-140-0_2",
language = "English",
volume = "948",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "13--22",
booktitle = "Nanotechnology for Nucleic Acid Delivery",

}

RIS

TY - CHAP

T1 - Lactate dehydrogenase assay for assessment of polycation cytotoxicity

AU - Parhamifar, Ladan

AU - Andersen, Helene

AU - Moghimi, Seyed Moien

PY - 2013

Y1 - 2013

N2 - Cellular toxicity and/or cell death entail complex mechanisms that require detailed evaluation for proper characterization. A detailed mechanistic assessment of cytotoxicity is essential for design and construction of more effective polycations for nucleic acid delivery. A single toxicity assay cannot stand alone in determining the type and extent of damage or cell death mechanism. In this chapter we describe a lactate dehydrogenase (LDH) assay for high-throughput screening that can be used as a starting point for further detailed cytotoxicity determination. LDH release is considered an early event in necrosis but a late event in apoptosis. An accurate temporal assessment of the toxic responses is crucial as late apoptosis may convert into necrosis as well as in situations where cell death is initiated without any visible cell morphological changes or responses in assays measuring late events, resulting in early ongoing toxicity being overlooked.

AB - Cellular toxicity and/or cell death entail complex mechanisms that require detailed evaluation for proper characterization. A detailed mechanistic assessment of cytotoxicity is essential for design and construction of more effective polycations for nucleic acid delivery. A single toxicity assay cannot stand alone in determining the type and extent of damage or cell death mechanism. In this chapter we describe a lactate dehydrogenase (LDH) assay for high-throughput screening that can be used as a starting point for further detailed cytotoxicity determination. LDH release is considered an early event in necrosis but a late event in apoptosis. An accurate temporal assessment of the toxic responses is crucial as late apoptosis may convert into necrosis as well as in situations where cell death is initiated without any visible cell morphological changes or responses in assays measuring late events, resulting in early ongoing toxicity being overlooked.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1007/978-1-62703-140-0_2

DO - 10.1007/978-1-62703-140-0_2

M3 - Book chapter

C2 - 23070760

VL - 948

T3 - Methods in Molecular Biology

SP - 13

EP - 22

BT - Nanotechnology for Nucleic Acid Delivery

ER -

ID: 44038020