Investigation of the introduction and dissemination of vanB Enterococcus faecium in the Capital Region of Denmark and development of a rapid and accurate clone-specific vanB E. faecium PCR

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Investigation of the introduction and dissemination of vanB Enterococcus faecium in the Capital Region of Denmark and development of a rapid and accurate clone-specific vanB E. faecium PCR. / Pinholt, Mette; Mollerup, Sarah; Boye, Kit; Worning, Peder; Holzknecht, Barbara Juliane; Nygaard, Sanne; Nielsen, Karen Leth; Hasman, Henrik; Roer, Louise; Hammerum, Anette M.; Westh, Henrik; Schønning, Kristian.

In: Journal of Antimicrobial Chemotherapy, Vol. 76, No. 9, 2021, p. 2260-2267.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pinholt, M, Mollerup, S, Boye, K, Worning, P, Holzknecht, BJ, Nygaard, S, Nielsen, KL, Hasman, H, Roer, L, Hammerum, AM, Westh, H & Schønning, K 2021, 'Investigation of the introduction and dissemination of vanB Enterococcus faecium in the Capital Region of Denmark and development of a rapid and accurate clone-specific vanB E. faecium PCR', Journal of Antimicrobial Chemotherapy, vol. 76, no. 9, pp. 2260-2267. https://doi.org/10.1093/jac/dkab198

APA

Pinholt, M., Mollerup, S., Boye, K., Worning, P., Holzknecht, B. J., Nygaard, S., Nielsen, K. L., Hasman, H., Roer, L., Hammerum, A. M., Westh, H., & Schønning, K. (2021). Investigation of the introduction and dissemination of vanB Enterococcus faecium in the Capital Region of Denmark and development of a rapid and accurate clone-specific vanB E. faecium PCR. Journal of Antimicrobial Chemotherapy, 76(9), 2260-2267. https://doi.org/10.1093/jac/dkab198

Vancouver

Pinholt M, Mollerup S, Boye K, Worning P, Holzknecht BJ, Nygaard S et al. Investigation of the introduction and dissemination of vanB Enterococcus faecium in the Capital Region of Denmark and development of a rapid and accurate clone-specific vanB E. faecium PCR. Journal of Antimicrobial Chemotherapy. 2021;76(9):2260-2267. https://doi.org/10.1093/jac/dkab198

Author

Pinholt, Mette ; Mollerup, Sarah ; Boye, Kit ; Worning, Peder ; Holzknecht, Barbara Juliane ; Nygaard, Sanne ; Nielsen, Karen Leth ; Hasman, Henrik ; Roer, Louise ; Hammerum, Anette M. ; Westh, Henrik ; Schønning, Kristian. / Investigation of the introduction and dissemination of vanB Enterococcus faecium in the Capital Region of Denmark and development of a rapid and accurate clone-specific vanB E. faecium PCR. In: Journal of Antimicrobial Chemotherapy. 2021 ; Vol. 76, No. 9. pp. 2260-2267.

Bibtex

@article{2ca370cda5af44c684ab9bb5b12295e9,

title = "Investigation of the introduction and dissemination of vanB Enterococcus faecium in the Capital Region of Denmark and development of a rapid and accurate clone-specific vanB E. faecium PCR",

abstract = "Background: During 2018-19, an increase of vanB vancomycin-resistant Enterococcus faecium (VREfm) was observed in the Capital Region of Denmark. vanA/vanB PCR performed directly on rectal swabs is accurate in detection of vanA; however, the positive predictive value for vanB-positive samples is low because of the presence of vanB in non-enterococcal gut commensals. Objectives: We investigated the epidemiology and clonal relatedness of vanB VREfm from the period 2015-19 and describe the application of a clone-specific vanB VREfm PCR assay for rapid and accurate detection of vanB VREfm in rectal screening samples. Methods: vanB VREfm were investigated using epidemiological data and WGS data. The SeqSphere+ software was used to analyse MLST and cgMLST, and de novo assemblies were annotated to determine insertion sites for the vanB transposon (Tn1549). A clone-specific vanB VREfm PCR assay was designed to detect the sequence bridging Tn1549 and the E. faecium chromosome (araA2) in the dominant cluster. Results: Two hundred and seventy-five vanB VREfm isolates were identified, of which 76% were identified in 2019. A dominant cluster (Cluster 1, n=204, 74%), six minor clusters and 15 singletons were identified. All Cluster 1 isolates and six non-Cluster 1 isolates had Tn1549 integrated into araA2. In 2019, the PCR assay would have detected 92% of all rectal screening samples containing vanB VREfm. Conclusions: vanB VREfm increased due to the introduction and nosocomial transmission of the successful Cluster 1. The clone-specific PCR assay detected vanB VREfm outbreak isolates in rectal screening samples rapidly and accurately. ",

author = "Mette Pinholt and Sarah Mollerup and Kit Boye and Peder Worning and Holzknecht, {Barbara Juliane} and Sanne Nygaard and Nielsen, {Karen Leth} and Henrik Hasman and Louise Roer and Hammerum, {Anette M.} and Henrik Westh and Kristian Sch{\o}nning",

year = "2021",

doi = "10.1093/jac/dkab198",

language = "English",

volume = "76",

pages = "2260--2267",

journal = "Journal of Antimicrobial Chemotherapy",

issn = "0305-7453",

publisher = "Oxford University Press",

number = "9",

}

RIS

TY - JOUR

T1 - Investigation of the introduction and dissemination of vanB Enterococcus faecium in the Capital Region of Denmark and development of a rapid and accurate clone-specific vanB E. faecium PCR

AU - Pinholt, Mette

AU - Mollerup, Sarah

AU - Boye, Kit

AU - Worning, Peder

AU - Holzknecht, Barbara Juliane

AU - Nygaard, Sanne

AU - Nielsen, Karen Leth

AU - Hasman, Henrik

AU - Roer, Louise

AU - Hammerum, Anette M.

AU - Westh, Henrik

AU - Schønning, Kristian

PY - 2021

Y1 - 2021

N2 - Background: During 2018-19, an increase of vanB vancomycin-resistant Enterococcus faecium (VREfm) was observed in the Capital Region of Denmark. vanA/vanB PCR performed directly on rectal swabs is accurate in detection of vanA; however, the positive predictive value for vanB-positive samples is low because of the presence of vanB in non-enterococcal gut commensals. Objectives: We investigated the epidemiology and clonal relatedness of vanB VREfm from the period 2015-19 and describe the application of a clone-specific vanB VREfm PCR assay for rapid and accurate detection of vanB VREfm in rectal screening samples. Methods: vanB VREfm were investigated using epidemiological data and WGS data. The SeqSphere+ software was used to analyse MLST and cgMLST, and de novo assemblies were annotated to determine insertion sites for the vanB transposon (Tn1549). A clone-specific vanB VREfm PCR assay was designed to detect the sequence bridging Tn1549 and the E. faecium chromosome (araA2) in the dominant cluster. Results: Two hundred and seventy-five vanB VREfm isolates were identified, of which 76% were identified in 2019. A dominant cluster (Cluster 1, n=204, 74%), six minor clusters and 15 singletons were identified. All Cluster 1 isolates and six non-Cluster 1 isolates had Tn1549 integrated into araA2. In 2019, the PCR assay would have detected 92% of all rectal screening samples containing vanB VREfm. Conclusions: vanB VREfm increased due to the introduction and nosocomial transmission of the successful Cluster 1. The clone-specific PCR assay detected vanB VREfm outbreak isolates in rectal screening samples rapidly and accurately.

AB - Background: During 2018-19, an increase of vanB vancomycin-resistant Enterococcus faecium (VREfm) was observed in the Capital Region of Denmark. vanA/vanB PCR performed directly on rectal swabs is accurate in detection of vanA; however, the positive predictive value for vanB-positive samples is low because of the presence of vanB in non-enterococcal gut commensals. Objectives: We investigated the epidemiology and clonal relatedness of vanB VREfm from the period 2015-19 and describe the application of a clone-specific vanB VREfm PCR assay for rapid and accurate detection of vanB VREfm in rectal screening samples. Methods: vanB VREfm were investigated using epidemiological data and WGS data. The SeqSphere+ software was used to analyse MLST and cgMLST, and de novo assemblies were annotated to determine insertion sites for the vanB transposon (Tn1549). A clone-specific vanB VREfm PCR assay was designed to detect the sequence bridging Tn1549 and the E. faecium chromosome (araA2) in the dominant cluster. Results: Two hundred and seventy-five vanB VREfm isolates were identified, of which 76% were identified in 2019. A dominant cluster (Cluster 1, n=204, 74%), six minor clusters and 15 singletons were identified. All Cluster 1 isolates and six non-Cluster 1 isolates had Tn1549 integrated into araA2. In 2019, the PCR assay would have detected 92% of all rectal screening samples containing vanB VREfm. Conclusions: vanB VREfm increased due to the introduction and nosocomial transmission of the successful Cluster 1. The clone-specific PCR assay detected vanB VREfm outbreak isolates in rectal screening samples rapidly and accurately.

U2 - 10.1093/jac/dkab198

DO - 10.1093/jac/dkab198

M3 - Journal article

C2 - 34151364

AN - SCOPUS:85114300570

VL - 76

SP - 2260

EP - 2267

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 9

ER -

ID: 279820457