Investigating Internalization and Intracellular Trafficking of GPCRs: New Techniques and Real-Time Experimental Approaches
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
The ability to regulate the interaction between cells and their extracellular environment is essential for the maintenance of appropriate physiological function. For G protein-coupled receptors (GPCRs), this regulation occurs through multiple mechanisms that provide spatial and temporal control for signal transduction. One of the major mechanisms for GPCR regulation involves their endocytic trafficking, which serves to internalize the receptors from the plasma membrane and thereby attenuate G protein-dependent signaling. However, there is accumulating evidence to suggest that GPCRs can signal independently of G proteins, as well as from intracellular compartments including endosomes. It is in this context that receptor internalization and intracellular trafficking have attracted renewed interest within the GPCR field. In this chapter, we will review the current understanding and methodologies that have been used to investigate internalization and intracellular signaling of GPCRs, with a particular focus on emerging real-time techniques. These recent developments have improved our understanding of the complexities of GPCR internalization and intracellular signaling and suggest that the broader biological relevance and potential therapeutic implications of these processes remain to be explored.
Original language | English |
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Title of host publication | Handbook of Experimental Pharmacology |
Volume | 245 |
Publisher | Springer |
Publication date | 11 Oct 2017 |
Pages | 41-61 |
DOIs | |
Publication status | Published - 11 Oct 2017 |
Series | Handbook of Experimental Pharmacology |
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ISSN | 0171-2004 |
- Journal Article
Research areas
ID: 186875910