Increased expression of glucagon-like peptide-1 receptors in psoriasis plaques

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Increased expression of glucagon-like peptide-1 receptors in psoriasis plaques. / Faurschou, Annesofie; Pedersen, Jens; Gyldenløve, Mette; Poulsen, Steen Seier; Holst, Jens J; Thyssen, Jacob P; Zachariae, Claus; Vilsbøll, Tina; Skov, Lone; Knop, Filip K.

In: Experimental Dermatology, Vol. 22, No. 2, 02.2013, p. 150-2.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Faurschou, A, Pedersen, J, Gyldenløve, M, Poulsen, SS, Holst, JJ, Thyssen, JP, Zachariae, C, Vilsbøll, T, Skov, L & Knop, FK 2013, 'Increased expression of glucagon-like peptide-1 receptors in psoriasis plaques', Experimental Dermatology, vol. 22, no. 2, pp. 150-2. https://doi.org/10.1111/exd.12081

APA

Faurschou, A., Pedersen, J., Gyldenløve, M., Poulsen, S. S., Holst, J. J., Thyssen, J. P., Zachariae, C., Vilsbøll, T., Skov, L., & Knop, F. K. (2013). Increased expression of glucagon-like peptide-1 receptors in psoriasis plaques. Experimental Dermatology, 22(2), 150-2. https://doi.org/10.1111/exd.12081

Vancouver

Faurschou A, Pedersen J, Gyldenløve M, Poulsen SS, Holst JJ, Thyssen JP et al. Increased expression of glucagon-like peptide-1 receptors in psoriasis plaques. Experimental Dermatology. 2013 Feb;22(2):150-2. https://doi.org/10.1111/exd.12081

Author

Faurschou, Annesofie ; Pedersen, Jens ; Gyldenløve, Mette ; Poulsen, Steen Seier ; Holst, Jens J ; Thyssen, Jacob P ; Zachariae, Claus ; Vilsbøll, Tina ; Skov, Lone ; Knop, Filip K. / Increased expression of glucagon-like peptide-1 receptors in psoriasis plaques. In: Experimental Dermatology. 2013 ; Vol. 22, No. 2. pp. 150-2.

Bibtex

@article{b6444814215f4dcbb1107f8ea9cc198f,

title = "Increased expression of glucagon-like peptide-1 receptors in psoriasis plaques",

abstract = "Recent case reports suggest that treatment with glucagon-like peptide-1 (GLP-1) agonists results in clinical improvement of psoriasis. The purpose of this study was to determine whether GLP-1 receptors (GLP-1Rs) are found in the skin of healthy volunteers and psoriasis patients and if so, whether GLP-1Rs are located on keratinocytes or immune cells. Three mm-punch skin biopsies were taken for gene expression analysis from six healthy volunteers and from affected and unaffected skin of six psoriasis patients. In addition, a blood sample was obtained from all participants. Cultured human keratinocytes were either untreated or incubated with tumor necrosis factor- a (TNF-a), interferon-¿ (IFN-¿) or a combination of TNF-a and IFN-¿ for 48 h. Total RNA was extracted from all the samples, reversely transcribed and analysed for the expression of GLP-1R using real-time PCR. Gene expression analysis showed expression of GLP-1Rs in five of six skin biopsies from psoriasis plaques, in one of six biopsies from unaffected psoriatic skin and in one of six biopsies from healthy skin. GLP-1R expression was found in the blood of both healthy volunteers and psoriasis patients. No GLP-1R expression was found in either stimulated or unstimulated cultured human keratinocytes. Our results show increased presence of GLP-1Rs in psoriasis plaques and that this most likely is due to infiltration with immune cells. This offers a possible explanation for the positive effect of treatment with GLP-1R agonists in patients with psoriasis.",

author = "Annesofie Faurschou and Jens Pedersen and Mette Gyldenl{\o}ve and Poulsen, {Steen Seier} and Holst, {Jens J} and Thyssen, {Jacob P} and Claus Zachariae and Tina Vilsb{\o}ll and Lone Skov and Knop, {Filip K}",

note = "{\textcopyright} 2013 John Wiley & Sons A/S.",

year = "2013",

month = feb,

doi = "10.1111/exd.12081",

language = "English",

volume = "22",

pages = "150--2",

journal = "Experimental Dermatology",

issn = "0906-6705",

publisher = "Wiley-Blackwell",

number = "2",

}

RIS

TY - JOUR

T1 - Increased expression of glucagon-like peptide-1 receptors in psoriasis plaques

AU - Faurschou, Annesofie

AU - Pedersen, Jens

AU - Gyldenløve, Mette

AU - Poulsen, Steen Seier

AU - Holst, Jens J

AU - Thyssen, Jacob P

AU - Zachariae, Claus

AU - Vilsbøll, Tina

AU - Skov, Lone

AU - Knop, Filip K

PY - 2013/2

Y1 - 2013/2

N2 - Recent case reports suggest that treatment with glucagon-like peptide-1 (GLP-1) agonists results in clinical improvement of psoriasis. The purpose of this study was to determine whether GLP-1 receptors (GLP-1Rs) are found in the skin of healthy volunteers and psoriasis patients and if so, whether GLP-1Rs are located on keratinocytes or immune cells. Three mm-punch skin biopsies were taken for gene expression analysis from six healthy volunteers and from affected and unaffected skin of six psoriasis patients. In addition, a blood sample was obtained from all participants. Cultured human keratinocytes were either untreated or incubated with tumor necrosis factor- a (TNF-a), interferon-¿ (IFN-¿) or a combination of TNF-a and IFN-¿ for 48 h. Total RNA was extracted from all the samples, reversely transcribed and analysed for the expression of GLP-1R using real-time PCR. Gene expression analysis showed expression of GLP-1Rs in five of six skin biopsies from psoriasis plaques, in one of six biopsies from unaffected psoriatic skin and in one of six biopsies from healthy skin. GLP-1R expression was found in the blood of both healthy volunteers and psoriasis patients. No GLP-1R expression was found in either stimulated or unstimulated cultured human keratinocytes. Our results show increased presence of GLP-1Rs in psoriasis plaques and that this most likely is due to infiltration with immune cells. This offers a possible explanation for the positive effect of treatment with GLP-1R agonists in patients with psoriasis.

AB - Recent case reports suggest that treatment with glucagon-like peptide-1 (GLP-1) agonists results in clinical improvement of psoriasis. The purpose of this study was to determine whether GLP-1 receptors (GLP-1Rs) are found in the skin of healthy volunteers and psoriasis patients and if so, whether GLP-1Rs are located on keratinocytes or immune cells. Three mm-punch skin biopsies were taken for gene expression analysis from six healthy volunteers and from affected and unaffected skin of six psoriasis patients. In addition, a blood sample was obtained from all participants. Cultured human keratinocytes were either untreated or incubated with tumor necrosis factor- a (TNF-a), interferon-¿ (IFN-¿) or a combination of TNF-a and IFN-¿ for 48 h. Total RNA was extracted from all the samples, reversely transcribed and analysed for the expression of GLP-1R using real-time PCR. Gene expression analysis showed expression of GLP-1Rs in five of six skin biopsies from psoriasis plaques, in one of six biopsies from unaffected psoriatic skin and in one of six biopsies from healthy skin. GLP-1R expression was found in the blood of both healthy volunteers and psoriasis patients. No GLP-1R expression was found in either stimulated or unstimulated cultured human keratinocytes. Our results show increased presence of GLP-1Rs in psoriasis plaques and that this most likely is due to infiltration with immune cells. This offers a possible explanation for the positive effect of treatment with GLP-1R agonists in patients with psoriasis.

U2 - 10.1111/exd.12081

DO - 10.1111/exd.12081

M3 - Journal article

C2 - 23362875

VL - 22

SP - 150

EP - 152

JO - Experimental Dermatology

JF - Experimental Dermatology

SN - 0906-6705

IS - 2

ER -

ID: 45047726