In vitro propagation and dynamics of T cells from skin biopsies by methods using interleukins-2 and -4 or anti-CD3/CD28 antibody-coated microbeads
Research output: Contribution to journal › Journal article › Research › peer-review
In order to explore the mechanisms of inflammatory skin disorders, we established two methods of expanding skin-derived lymphocytes, one using high levels of interleukin (IL)-2 and IL-4 (method A) and the other using low levels of cytokines and anti-CD3/CD28 microbeads (method B). Both methods provide advantages for functional studies. With either of these two, we could obtain more than 10(7) cells/ from a 3 mm skin biopsy in 21 days from 23 out of 26 biopsies of various skin diseases. The relevance of these cells was confirmed by shifted T-cell receptor beta chain variable region (TCR-Vbeta) repertoire and antigen-dependent proliferation in antigen-driven skin disorders. The propagation of skin-resident lymphocytes, seen especially in method A, seems to be mediated by a functional defect of regulatory T cells residing in skin sequentially expanding under the conditions of our methods.
Original language | English |
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Journal | Acta Dermato Venereologica |
Volume | 90 |
Issue number | 5 |
Pages (from-to) | 468-73 |
Number of pages | 6 |
ISSN | 0001-5555 |
DOIs | |
Publication status | Published - 1 Sep 2010 |
- Adult, Aged, Antibodies, Antigens, CD28, Antigens, CD3, Biopsy, Cell Culture Techniques, Cell Line, Cell Proliferation, Cell Separation, Cytotoxicity, Immunologic, Denmark, Female, Flow Cytometry, Humans, Interleukin-2, Interleukin-4, Japan, Male, Microspheres, Middle Aged, Phenotype, Receptors, Antigen, T-Cell, alpha-beta, Recombinant Proteins, Skin, T-Lymphocytes, Time Factors
Research areas
ID: 33587124