IL-33 induces IL-9 production in human CD4+ T cells and basophils
Research output: Contribution to journal › Journal article › Research › peer-review
Documents
- IL-33 Induces IL-9 Production in Human CD4+ T Cells and Basophils
Final published version, 888 KB, PDF document
IL-33, an IL-1 family member and ligand for the IL-1 receptor-related protein ST2, has been associated with induction of Th2 cytokines such as IL-4, IL-5, and IL-13. Here, we report that IL-33 can initiate IL-9 protein secretion in vitro in human CD4+ T cells and basophils isolated from peripheral blood. TGF-β has been described as a critical factor for IL-9 induction in Th2 cells; however, we found that TGF-β also induces co-production of IL-9 in purified, naïve (>99%) CD4(+)CD45RA(+)CD45RO(-)CD25(-) T cells differentiated towards a Th1 profile. Subsequently, it was demonstrated that TGF-β is important, although not an absolute requirement, for IL-9 production in CD4+ T cells. IL-9 production by purified (>95%) human basophils, cultured for 24 h with IL-3 or IL-33, was found, with a strong synergy between the two, likely to be explained by the IL-3 upregulated ST2 expression. Collectively, these data indicate that barrier functioning cells are important for the regulation of IL-9 production by immune cells in inflamed tissue.
Original language | English |
---|---|
Article number | e21695 |
Journal | P L o S One |
Volume | 6 |
Issue number | 7 |
Number of pages | 11 |
ISSN | 1932-6203 |
DOIs | |
Publication status | Published - 2011 |
- Basophils, CD4-Positive T-Lymphocytes, Cell Separation, Gene Expression Regulation, Humans, Interleukin-4, Interleukin-9, Interleukins, RNA, Messenger, Receptors, Cell Surface, Th1 Cells, Th2 Cells, Transforming Growth Factor beta
Research areas
Number of downloads are based on statistics from Google Scholar and www.ku.dk
No data available
ID: 33729868